TY - JOUR
T1 - Aspiration-assisted bioprinting for precise positioning of biologics
AU - Ayan, Bugra
AU - Heo, Dong Nyoung
AU - Zhang, Zhifeng
AU - Dey, Madhuri
AU - Povilianskas, Adomas
AU - Drapaca, Corina
AU - Ozbolat, Ibrahim T.
N1 - Funding Information:
Acknowledgments: We thank J. Gallant from Michigan State University for providing electric fish and B. Cheng from PSU for providing a high-speed camera for contact angle measurements. We thank Y. Wu, D. Sosnoski, D. N. Branford, and M. A. Alioglu from PSU for assistance in providing tissue strands, culturing cells, designing the graphics for Fig. 1, and maintaining electric fish, respectively. We are also grateful to D. Ravnic and S. Koduru (Department of Surgery at PSU) for providing ADSCs, N. Zavazava (Department of Internal Medicine at the University of Iowa) for providing HDFs, and A. Mastro (Department of Biochemistry and Molecular Biology at PSU) for providing 3T3 and 4T1 cells. We thank RoosterBio for providing MSCs and GM. We also acknowledge the support from The Huck Institutes of Life Sciences and Materials Research Institute for providing facilities for characterization of experiments. Funding: This work has been supported by NSF awards 1914885 (I.T.O.) and 1624515 (I.T.O.), NIH award R21 CA224422 01A1 (I.T.O.), and a Convergence grant from the Materials Research Institute at PSU (I.T.O.). B.A. acknowledges the support from the Turkish Ministry of National Education.
Publisher Copyright:
© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
PY - 2020
Y1 - 2020
N2 - Three-dimensional (3D) bioprinting is an appealing approach for building tissues; however, bioprinting of mini-tissue blocks (i.e., spheroids) with precise control on their positioning in 3D space has been a major obstacle. Here, we unveil “aspiration-assisted bioprinting (AAB),” which enables picking and bioprinting biologics in 3D through harnessing the power of aspiration forces, and when coupled with microvalve bioprinting, it facilitated different biofabrication schemes including scaffold-based or scaffold-free bioprinting at an unprecedented placement precision, ~11% with respect to the spheroid size. We studied the underlying physical mechanism of AAB to understand interactions between aspirated viscoelastic spheroids and physical governing forces during aspiration and bioprinting. We bioprinted a wide range of biologics with dimensions in an order-of-magnitude range including tissue spheroids (80 to 600 μm), tissue strands (~800 μm), or single cells (electrocytes, ~400 μm), and as applications, we illustrated the patterning of angiogenic sprouting spheroids and self-assembly of osteogenic spheroids.
AB - Three-dimensional (3D) bioprinting is an appealing approach for building tissues; however, bioprinting of mini-tissue blocks (i.e., spheroids) with precise control on their positioning in 3D space has been a major obstacle. Here, we unveil “aspiration-assisted bioprinting (AAB),” which enables picking and bioprinting biologics in 3D through harnessing the power of aspiration forces, and when coupled with microvalve bioprinting, it facilitated different biofabrication schemes including scaffold-based or scaffold-free bioprinting at an unprecedented placement precision, ~11% with respect to the spheroid size. We studied the underlying physical mechanism of AAB to understand interactions between aspirated viscoelastic spheroids and physical governing forces during aspiration and bioprinting. We bioprinted a wide range of biologics with dimensions in an order-of-magnitude range including tissue spheroids (80 to 600 μm), tissue strands (~800 μm), or single cells (electrocytes, ~400 μm), and as applications, we illustrated the patterning of angiogenic sprouting spheroids and self-assembly of osteogenic spheroids.
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U2 - 10.1126/sciadv.aaw5111
DO - 10.1126/sciadv.aaw5111
M3 - Article
C2 - 32181332
AN - SCOPUS:85081947108
SN - 2375-2548
VL - 6
JO - Science Advances
JF - Science Advances
IS - 10
M1 - eaaw5111
ER -