Assembly of combinatorial antibody libraries on phage surfaces: The gene III site

Carlos F. Barbas; Anoray S. Kang; Richard A. Lerner; Stephen J. Benkovic

doi:10.1073/pnas.88.18.7978

Assembly of combinatorial antibody libraries on phage surfaces: The gene III site

Carlos F. Barbas, Anoray S. Kang, Richard A. Lerner, Stephen J. Benkovic

Chemistry

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1078 Scopus citations

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 10⁵-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

Original language	English (US)
Pages (from-to)	7978-7982
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	18
DOIs	https://doi.org/10.1073/pnas.88.18.7978
State	Published - 1991

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abstract = "A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 105-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.",

author = "Barbas, {Carlos F.} and Kang, {Anoray S.} and Lerner, {Richard A.} and Benkovic, {Stephen J.}",

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T1 - Assembly of combinatorial antibody libraries on phage surfaces

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AU - Kang, Anoray S.

AU - Lerner, Richard A.

AU - Benkovic, Stephen J.

PY - 1991

Y1 - 1991

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AB - A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 105-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Assembly of combinatorial antibody libraries on phage surfaces: The gene III site

Abstract

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