Assembly of combinatorial antibody libraries on phage surfaces: The gene III site

Carlos F. Barbas; Anoray S. Kang; Richard A. Lerner; Stephen J. Benkovic

doi:10.1073/pnas.88.18.7978

Assembly of combinatorial antibody libraries on phage surfaces: The gene III site

Carlos F. Barbas
, Anoray S. Kang
, Richard A. Lerner
, Stephen J. Benkovic

Chemistry

Research output: Contribution to journal › Article › peer-review

1124 Scopus citations

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 10⁵-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

Original language	English (US)
Pages (from-to)	7978-7982
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	18
DOIs	https://doi.org/10.1073/pnas.88.18.7978
State	Published - 1991

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title = "Assembly of combinatorial antibody libraries on phage surfaces: The gene III site",

abstract = "A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 105-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.",

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AU - Lerner, Richard A.

AU - Benkovic, Stephen J.

PY - 1991

Y1 - 1991

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AB - A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carbozyl-terminal domain of the gene in protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 103- to 105-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

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AN - SCOPUS:0025838021

SN - 0027-8424

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JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 18

ER -

Assembly of combinatorial antibody libraries on phage surfaces: The gene III site

Abstract

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