Assessing ballast water treatments: Evaluation of viability methods for ambient freshwater microplankton assemblages

Euan D. Reavie, Allegra A. Cangelosi, Lisa E. Allinger

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


For decades the Great Lakes have been subject to invasive species introductions through the discharge of ships' ballast water. Several treatment technologies involving physical, chemical, and biological processes have been developed to remove or inactivate organisms in this discharge. Assessing the efficacy of these technologies involves estimating the number of viable propagules in treated discharge relative to untreated controls. For organisms in the 10-50μm size range, for example, the International Maritime Organization (IMO) mandates that fewer than 10 viable organisms per milliliter may be discharged. To date, however, there is no standard method to assess viability of natural assemblages of organisms in this size group (largely phytoplankton and protozoans) in freshwater environments. We report here on a process of assemblage concentration, staining with fluorescein diacetate (FDA), and microscopic observation as a reliable and efficient method to assess densities of viable freshwater organisms in this size category in ballast discharge. A number of other methods, including digestion with enzymes, flow cytometry, and a variety of vital and mortal stains, were tested and discarded during this vetting process due to inconsistent or ambiguous results.

Original languageEnglish (US)
Pages (from-to)540-547
Number of pages8
JournalJournal of Great Lakes Research
Issue number3
StatePublished - Sep 2010

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology


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