TY - GEN
T1 - Assessing human exposure to polycyclic aromatic hydrocarbons by urinary biomonitoring
AU - Li, Zheng
AU - Romanoff, Lovisa C.
AU - Trinidad, Debra A.
AU - Hussain, Narisa
AU - Jones, Richard S.
AU - Porter, Erin N.
AU - Needham, Larry L.
AU - Patterson, Donald G.
AU - Sjodin, Andreas
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - Polycyclic aromatic hydrocarbons (PAHs), a class of carcinogenic and mutagenic pollutants formed during incomplete combustion processes, are found in automobile exhausts, forest fires, cigarette smoke and certain foods such as charbroiled meat. Human exposure to environmental PAHs can be assessed by measuring levels of their urinary metabolites, hydroxy-PAHs (OH-PAHs). We have developed a method for the measurement of 24 OH-PAHs in urine. The method consists of enzymatic hydrolysis, automated liquid-liquid extraction, derivatization and quantification by gas chromatography/isotope dilution high-resolution mass spectrometry (GC/IDHRMS). The limits of detection were in the low parts-per-trillion range. The CVs were 2.9-11%, method recoveries were 46-72% and the throughput was 40 samples per day per analyst. This method has been applied to the analysis of samples from the National Health and Nutrition Examination Survey (NHANES), performed by CDC to assess exposure of the U.S. general population to environmental pollutants.
AB - Polycyclic aromatic hydrocarbons (PAHs), a class of carcinogenic and mutagenic pollutants formed during incomplete combustion processes, are found in automobile exhausts, forest fires, cigarette smoke and certain foods such as charbroiled meat. Human exposure to environmental PAHs can be assessed by measuring levels of their urinary metabolites, hydroxy-PAHs (OH-PAHs). We have developed a method for the measurement of 24 OH-PAHs in urine. The method consists of enzymatic hydrolysis, automated liquid-liquid extraction, derivatization and quantification by gas chromatography/isotope dilution high-resolution mass spectrometry (GC/IDHRMS). The limits of detection were in the low parts-per-trillion range. The CVs were 2.9-11%, method recoveries were 46-72% and the throughput was 40 samples per day per analyst. This method has been applied to the analysis of samples from the National Health and Nutrition Examination Survey (NHANES), performed by CDC to assess exposure of the U.S. general population to environmental pollutants.
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M3 - Conference contribution
AN - SCOPUS:34047269625
SN - 0841274266
SN - 9780841274266
T3 - ACS National Meeting Book of Abstracts
BT - Abstracts of Papers - 232nd American Chemical Society Meeting and Exposition
T2 - 232nd American Chemical Society Meeting and Exposition
Y2 - 10 September 2006 through 14 September 2006
ER -
