Assessment of bulking strategies for RAPD analyses of flax germplasm

Yong Bi Fu; Sarah Guerin; Gregor W. Peterson; John E. Carlson; K. W. Richards

doi:10.1023/A:1025015401382

Assessment of bulking strategies for RAPD analyses of flax germplasm

Yong Bi Fu, Sarah Guerin, Gregor W. Peterson, John E. Carlson, K. W. Richards

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17 Scopus citations

Abstract

Effectiveness of several bulking strategies was empirically assessed in detecting RAPD variations and determining genetic relationships of five flax (Linum usitatissimum L.) landrace accessions. Bulking ten individuals before and after DNA isolations generated consistent RAPD variations. About 30% of the polymorphic RAPD loci observed in the plant-by-plant (PBP) sample were difficult to score and/or undetected in the bulked samples of the same accession. Heterogeneity among the six bulked samples of the same accession was observed at 5.6% of the loci scored. The frequency of a specific RAPD band present in those individuals used to form a bulk was at least 1/11 for its detection in the bulked sample. In spite of these limitations, bulking still generated compatible genetic relationships of the five accessions from its PBP sampling.

Original language	English (US)
Pages (from-to)	743-746
Number of pages	4
Journal	Genetic Resources and Crop Evolution
Volume	50
Issue number	7
DOIs	https://doi.org/10.1023/A:1025015401382
State	Published - Nov 2003

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Agronomy and Crop Science
Genetics
Plant Science

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title = "Assessment of bulking strategies for RAPD analyses of flax germplasm",

abstract = "Effectiveness of several bulking strategies was empirically assessed in detecting RAPD variations and determining genetic relationships of five flax (Linum usitatissimum L.) landrace accessions. Bulking ten individuals before and after DNA isolations generated consistent RAPD variations. About 30% of the polymorphic RAPD loci observed in the plant-by-plant (PBP) sample were difficult to score and/or undetected in the bulked samples of the same accession. Heterogeneity among the six bulked samples of the same accession was observed at 5.6% of the loci scored. The frequency of a specific RAPD band present in those individuals used to form a bulk was at least 1/11 for its detection in the bulked sample. In spite of these limitations, bulking still generated compatible genetic relationships of the five accessions from its PBP sampling.",

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T1 - Assessment of bulking strategies for RAPD analyses of flax germplasm

AU - Fu, Yong Bi

AU - Guerin, Sarah

AU - Peterson, Gregor W.

AU - Carlson, John E.

AU - Richards, K. W.

PY - 2003/11

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N2 - Effectiveness of several bulking strategies was empirically assessed in detecting RAPD variations and determining genetic relationships of five flax (Linum usitatissimum L.) landrace accessions. Bulking ten individuals before and after DNA isolations generated consistent RAPD variations. About 30% of the polymorphic RAPD loci observed in the plant-by-plant (PBP) sample were difficult to score and/or undetected in the bulked samples of the same accession. Heterogeneity among the six bulked samples of the same accession was observed at 5.6% of the loci scored. The frequency of a specific RAPD band present in those individuals used to form a bulk was at least 1/11 for its detection in the bulked sample. In spite of these limitations, bulking still generated compatible genetic relationships of the five accessions from its PBP sampling.

AB - Effectiveness of several bulking strategies was empirically assessed in detecting RAPD variations and determining genetic relationships of five flax (Linum usitatissimum L.) landrace accessions. Bulking ten individuals before and after DNA isolations generated consistent RAPD variations. About 30% of the polymorphic RAPD loci observed in the plant-by-plant (PBP) sample were difficult to score and/or undetected in the bulked samples of the same accession. Heterogeneity among the six bulked samples of the same accession was observed at 5.6% of the loci scored. The frequency of a specific RAPD band present in those individuals used to form a bulk was at least 1/11 for its detection in the bulked sample. In spite of these limitations, bulking still generated compatible genetic relationships of the five accessions from its PBP sampling.

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Assessment of bulking strategies for RAPD analyses of flax germplasm

Abstract

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