TY - JOUR
T1 - Association of blood lead levels with urinary F2-8α isoprostane and 8-hydroxy-2-deoxy-guanosine concentrations in first-grade Uruguayan children
AU - Roy, Aditi
AU - Queirolo, Elena
AU - Peregalli, Fabiana
AU - Mañay, Nelly
AU - Martínez, Gabriela
AU - Kordas, Katarzyna
N1 - Funding Information:
This study was supported by the U.S. National Institute of Environmental Health Sciences (NIEHS) grant 1R21 ES16523-01. The authors would like to thank pediatric nurses Ms. Delma Ribeiro and Ms. Graciela Yuane for conducting clinic visits, the nutritionists Ms. María Soledad Mangieri, Ms. Virginia Ocampo, Ms. Valentina Baccino and Ms. Elizabeth Barcia for collecting the dietary data and the other study staffs- Ms. Daniela Cicarriello, Ms. Natalia Agudelo, Ms. Jimena Deana, Ms. Marcedez Perez, Ms. Maria Sicardi, Ms. Lucia de Mattos and Ms. Marta Grundell who helped with the parental questionnaire administration. We would like to thank Dr. Erica Unger for providing space and guidance for biochemical analyses and Dr. Laura-Murray Kolb for advice on CRP measurements.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Oxidative stress (OS) is a potential molecular mechanism for lead-induced toxicities, yet, we have limited understanding of the relation between low-level lead (Pb) exposure and OS, especially in children. This cross-sectional study examines the association between blood lead level (BLL) and two OS markers-urinary F2-8α isoprostane or isoprostane (a marker of lipid peroxidation) and 8-hydroxy-2-deoxy-Guanosine or 8-OH-dG (a marker of DNA damage) in 211 children, aged 5-8 years, from Montevideo, Uruguay. The role of dietary intakes of vitamin C and zinc in modifying the relation between BLL and OS was also examined. The mean (SD) BLL of the study children was 4.7 (2.2)μg/dL, with 30.2% children having BLL ≥5μg/dL, the current reference level set by the US Centre for Disease Control for identifying, monitoring and management of children with elevated BLL. In covariate-adjusted analysis, there was a weak positive association between BLL and urinary isoprostane (adjusted for specific gravity) [β=0.09, p<0.1]. No association was found between children's BLL and urinary 8-OH-dG. Interactions between dietary intakes of vitamin C or zinc and BLL on OS biomarkers were not consistent. However, when BLL and vitamin C or BLL and zinc were modeled together, BLL was independently associated with isoprostane concentration [β=0.10, p<0.05] but vitamin C or zinc intake was not. These findings suggest that there may be a potential adverse effect of BLL on OS in children with low-level Pb exposure. There is a need to study the effects of Pb on other OS measures, as well as the role of OS in mediating low-level Pb toxicity on functional outcomes.
AB - Oxidative stress (OS) is a potential molecular mechanism for lead-induced toxicities, yet, we have limited understanding of the relation between low-level lead (Pb) exposure and OS, especially in children. This cross-sectional study examines the association between blood lead level (BLL) and two OS markers-urinary F2-8α isoprostane or isoprostane (a marker of lipid peroxidation) and 8-hydroxy-2-deoxy-Guanosine or 8-OH-dG (a marker of DNA damage) in 211 children, aged 5-8 years, from Montevideo, Uruguay. The role of dietary intakes of vitamin C and zinc in modifying the relation between BLL and OS was also examined. The mean (SD) BLL of the study children was 4.7 (2.2)μg/dL, with 30.2% children having BLL ≥5μg/dL, the current reference level set by the US Centre for Disease Control for identifying, monitoring and management of children with elevated BLL. In covariate-adjusted analysis, there was a weak positive association between BLL and urinary isoprostane (adjusted for specific gravity) [β=0.09, p<0.1]. No association was found between children's BLL and urinary 8-OH-dG. Interactions between dietary intakes of vitamin C or zinc and BLL on OS biomarkers were not consistent. However, when BLL and vitamin C or BLL and zinc were modeled together, BLL was independently associated with isoprostane concentration [β=0.10, p<0.05] but vitamin C or zinc intake was not. These findings suggest that there may be a potential adverse effect of BLL on OS in children with low-level Pb exposure. There is a need to study the effects of Pb on other OS measures, as well as the role of OS in mediating low-level Pb toxicity on functional outcomes.
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UR - http://www.scopus.com/inward/citedby.url?scp=84926201312&partnerID=8YFLogxK
U2 - 10.1016/j.envres.2015.03.001
DO - 10.1016/j.envres.2015.03.001
M3 - Article
C2 - 25863186
AN - SCOPUS:84926201312
SN - 0013-9351
VL - 140
SP - 127
EP - 135
JO - Environmental Research
JF - Environmental Research
ER -