TY - JOUR
T1 - Association of thioautotrophic bacteria with deep-sea sponges
AU - Nishijima, Miyuki
AU - Lindsay, Dhugal J.
AU - Hata, Junko
AU - Nakamura, Aoi
AU - Kasai, Hiroaki
AU - Ise, Yuji
AU - Fisher, Charles R.
AU - Fujiwara, Yoshihiro
AU - Kawato, Masaru
AU - Maruyama, Tadashi
N1 - Funding Information:
We would like to thank the captains and crews of the R/Vs Kaiyo and Seward Johnson and the operation teams of the Johnson Sea-Link submersible and of the ROV Hyper-Dolphin. Dr. David Julian of the University of Florida is acknowledged for collecting sponges in his dive, JSL no. 4583 in the Gulf of Mexico. We are grateful to Ms. Yukiko Itazawa for her skillful technical assistance. The research cruise in the Gulf of Mexico was supported by NSF OCE 0117050 and a grant from the US NOAA Ocean Exploration program to CRF.
PY - 2010/6
Y1 - 2010/6
N2 - We investigated microorganisms associated with a deep-sea sponge, Characella sp. (Pachastrellidae) collected at a hydrothermal vent site (686 m depth) in the Sumisu Caldera, Ogasawara Island chain, Japan, and with two sponges, Pachastrella sp. (Pachastrellidae) and an unidentified Poecilosclerida sponge, collected at an oil seep (572 m depth) in the Gulf of Mexico, using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) directed at bacterial 16S rRNA gene sequences. In the PCR-DGGE profiles, we detected a single clearly dominant band in each of the Characella sp. and the unidentified Poecilosclerida sponge. BLAST search of their sequences showed that they were most similar (>99% identity) to those of the gammaproteobacterial thioautotrophic symbionts of deep-sea bivalves from hydrothermal vents, Bathymodiolus spp. Phylogenetic analysis of the near-full length sequences of the 16S rRNA genes cloned from the unidentified Poecilosclerida sponge and Characella sp. confirmed that they were closely related to thioautotrophic symbionts. Although associations between sponges and methanotrophic bacteria have been reported previously, this is the first report of a possible stable association between sponges and thioautotrophic bacteria.
AB - We investigated microorganisms associated with a deep-sea sponge, Characella sp. (Pachastrellidae) collected at a hydrothermal vent site (686 m depth) in the Sumisu Caldera, Ogasawara Island chain, Japan, and with two sponges, Pachastrella sp. (Pachastrellidae) and an unidentified Poecilosclerida sponge, collected at an oil seep (572 m depth) in the Gulf of Mexico, using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) directed at bacterial 16S rRNA gene sequences. In the PCR-DGGE profiles, we detected a single clearly dominant band in each of the Characella sp. and the unidentified Poecilosclerida sponge. BLAST search of their sequences showed that they were most similar (>99% identity) to those of the gammaproteobacterial thioautotrophic symbionts of deep-sea bivalves from hydrothermal vents, Bathymodiolus spp. Phylogenetic analysis of the near-full length sequences of the 16S rRNA genes cloned from the unidentified Poecilosclerida sponge and Characella sp. confirmed that they were closely related to thioautotrophic symbionts. Although associations between sponges and methanotrophic bacteria have been reported previously, this is the first report of a possible stable association between sponges and thioautotrophic bacteria.
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U2 - 10.1007/s10126-009-9253-7
DO - 10.1007/s10126-009-9253-7
M3 - Article
C2 - 20221658
AN - SCOPUS:77953020945
SN - 1436-2228
VL - 12
SP - 253
EP - 260
JO - Marine Biotechnology
JF - Marine Biotechnology
IS - 3
ER -