Atrial natriuretic factor regulates steroidogenic responsiveness and cyclic nucleotide levels in mouse leydig cells in vitro

Kailash N. Pandey; Spyros N. Paylou; William Kovacs; Tadashi Inagami

doi:10.1016/0006-291X(86)90295-0

Atrial natriuretic factor regulates steroidogenic responsiveness and cyclic nucleotide levels in mouse leydig cells in vitro

Kailash N. Pandey, Spyros N. Paylou, William Kovacs, Tadashi Inagami

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Abstract

The effects of synthetic atrial natriuretic factor (ANF) on the regulation of mouse Leydig cell steroidogenesis have been studied in vitro. ANF in nanomolar concentration increased testosterone production by more than 30-fold over basal levels. Concomitantly, cyclic guanosine monophosphate levels were increased 35-fold; cyclic adenosine monophosphate levels fell minimally (15-20%). ANF at low concentration (1×10^-11 M) inhibited testosterone production by luteinizing hormone-stimulated cells, while at higher concentration (> 2×10^-9M) ANF stimulated steroidogenesis beyond the level attained by luteinizing hormone alone. These results indicate that ANF can exert stimulatory effects on testosterone steroidogenesis in vitro, and that the mechanism may involve an intracellular messenger other than cyclic adenosine monophosphate.

Original language	English (US)
Pages (from-to)	399-404
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	138
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(86)90295-0
State	Published - Jul 16 1986

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Atrial natriuretic factor regulates steroidogenic responsiveness and cyclic nucleotide levels in mouse leydig cells in vitro",

abstract = "The effects of synthetic atrial natriuretic factor (ANF) on the regulation of mouse Leydig cell steroidogenesis have been studied in vitro. ANF in nanomolar concentration increased testosterone production by more than 30-fold over basal levels. Concomitantly, cyclic guanosine monophosphate levels were increased 35-fold; cyclic adenosine monophosphate levels fell minimally (15-20%). ANF at low concentration (1×10-11 M) inhibited testosterone production by luteinizing hormone-stimulated cells, while at higher concentration (> 2×10-9M) ANF stimulated steroidogenesis beyond the level attained by luteinizing hormone alone. These results indicate that ANF can exert stimulatory effects on testosterone steroidogenesis in vitro, and that the mechanism may involve an intracellular messenger other than cyclic adenosine monophosphate.",

author = "Pandey, {Kailash N.} and Paylou, {Spyros N.} and William Kovacs and Tadashi Inagami",

note = "Funding Information: We acknowledge the This work was supported is Research Fellow of Chapter.",

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T1 - Atrial natriuretic factor regulates steroidogenic responsiveness and cyclic nucleotide levels in mouse leydig cells in vitro

AU - Pandey, Kailash N.

AU - Paylou, Spyros N.

AU - Kovacs, William

AU - Inagami, Tadashi

N1 - Funding Information: We acknowledge the This work was supported is Research Fellow of Chapter.

PY - 1986/7/16

Y1 - 1986/7/16

N2 - The effects of synthetic atrial natriuretic factor (ANF) on the regulation of mouse Leydig cell steroidogenesis have been studied in vitro. ANF in nanomolar concentration increased testosterone production by more than 30-fold over basal levels. Concomitantly, cyclic guanosine monophosphate levels were increased 35-fold; cyclic adenosine monophosphate levels fell minimally (15-20%). ANF at low concentration (1×10-11 M) inhibited testosterone production by luteinizing hormone-stimulated cells, while at higher concentration (> 2×10-9M) ANF stimulated steroidogenesis beyond the level attained by luteinizing hormone alone. These results indicate that ANF can exert stimulatory effects on testosterone steroidogenesis in vitro, and that the mechanism may involve an intracellular messenger other than cyclic adenosine monophosphate.

AB - The effects of synthetic atrial natriuretic factor (ANF) on the regulation of mouse Leydig cell steroidogenesis have been studied in vitro. ANF in nanomolar concentration increased testosterone production by more than 30-fold over basal levels. Concomitantly, cyclic guanosine monophosphate levels were increased 35-fold; cyclic adenosine monophosphate levels fell minimally (15-20%). ANF at low concentration (1×10-11 M) inhibited testosterone production by luteinizing hormone-stimulated cells, while at higher concentration (> 2×10-9M) ANF stimulated steroidogenesis beyond the level attained by luteinizing hormone alone. These results indicate that ANF can exert stimulatory effects on testosterone steroidogenesis in vitro, and that the mechanism may involve an intracellular messenger other than cyclic adenosine monophosphate.

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Atrial natriuretic factor regulates steroidogenic responsiveness and cyclic nucleotide levels in mouse leydig cells in vitro

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