TY - JOUR
T1 - Atypical Plant miRNA cal-miR2911
T2 - Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice
AU - Xu, Qin
AU - Wang, Jianing
AU - Zhang, Yi
AU - Li, Ying
AU - Qin, Xinshu
AU - Xin, Yirao
AU - Li, Yinglei
AU - Xu, Ke
AU - Yang, Xingbin
AU - Wang, Xingyu
N1 - Publisher Copyright:
© 2024 American Chemical Society
PY - 2024/3/6
Y1 - 2024/3/6
N2 - Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.
AB - Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.
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U2 - 10.1021/acs.jafc.3c09511
DO - 10.1021/acs.jafc.3c09511
M3 - Article
C2 - 38393993
AN - SCOPUS:85186208170
SN - 0021-8561
VL - 72
SP - 4801
EP - 4813
JO - Journal of agricultural and food chemistry
JF - Journal of agricultural and food chemistry
IS - 9
ER -