Bacteria require phase separation for fitness in the mammalian gut

Emilia Krypotou, Guy E. Townsend, Xiaohui Gao, Shoichi Tachiyama, Jun Liu, Nick D. Pokorzynski, Andrew L. Goodman, Eduardo A. Groisman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Therapeutic manipulation of the gut microbiota holds great potential for human health. The mechanisms bacteria use to colonize the gut therefore present valuable targets for clinical intervention. We now report that bacteria use phase separation to enhance fitness in the mammalian gut. We establish that the intrinsically disordered region (IDR) of the broadly and highly conserved transcription termination factor Rho is necessary and sufficient for phase separation in vivo and in vitro in the human commensal Bacteroides thetaiotaomicron. Phase separation increases transcription termination by Rho in an IDR-dependent manner. Moreover, the IDR is critical for gene regulation in the gut. Our findings expose phase separation as vital for host-commensal bacteria interactions and relevant for novel clinical applications.

Original languageEnglish (US)
Article numbereabn7229
JournalScience
Volume379
Issue number6637
DOIs
StatePublished - Mar 17 2023

All Science Journal Classification (ASJC) codes

  • General

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