TY - JOUR
T1 - Bacteriocinogenic potential of a probiotic strain Bacillus coagulans [BDU3] from Ngari
AU - Abdhul, Kaja
AU - Ganesh, Mohan
AU - Shanmughapriya, Santhanam
AU - Vanithamani, Shanmugam
AU - Kanagavel, Murugesan
AU - Anbarasu, Kumarasamy
AU - Natarajaseenivasan, Kalimuthusamy
N1 - Funding Information:
Authors greatly acknowledge Department of Biotechnology (DBT) and Department of Science and Technology (DST) , Government of India for funding. We acknowledge Indian Institute of Science Education and Research (IISER), Pune for their help in MALDI-TOF studies. We also extent our thanks to the Vice-Chancellor, Bharathidasan University and the Principal and Management, Nandha College for the facilities provided.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Bacteriocin producing strain BDU3 was isolated from a traditional fermented fish of Manipur Ngari. The strain BDU3 was identified as Bacillus coagulans by phenotypic and genotypic characterization. The BDU3 produced novel bacteriocin, which showed an antimicrobial spectrum toward a wide spectrum of food borne, and closely related pathogens with a MIC that ranged between 0.5 and 2.5μg/mL. The isolate was able to tolerate pH as low as 2.0 and up to 0.2% bile salt concentration. Three step purification was employed to increase the specific activity of the antimicrobial compound. The fractions were further chromatographed by Rp-HPLC C-18 column and the purified bacteriocin had a specific activity of ~8500AU/mg. However, the potency of bacteriocin was susceptible to digestion with Proteinase K, Pepsin, SDS, EDTA and Urea. Molecular mass of purified bacteriocin was found to be 1.4kDa using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). The functional group was revealed by FTIR analysis. The cytotoxicity assay (MTT) using purified bacteriocin showed 2 times lower EC50 values compared to SDS. This is the smaller bacteriocin ever reported before from B. coagulans with greater antimicrobial potency with lower cytotoxicity. This bacteriocin raises the possibilities to be used as a biopreservative in food industries.
AB - Bacteriocin producing strain BDU3 was isolated from a traditional fermented fish of Manipur Ngari. The strain BDU3 was identified as Bacillus coagulans by phenotypic and genotypic characterization. The BDU3 produced novel bacteriocin, which showed an antimicrobial spectrum toward a wide spectrum of food borne, and closely related pathogens with a MIC that ranged between 0.5 and 2.5μg/mL. The isolate was able to tolerate pH as low as 2.0 and up to 0.2% bile salt concentration. Three step purification was employed to increase the specific activity of the antimicrobial compound. The fractions were further chromatographed by Rp-HPLC C-18 column and the purified bacteriocin had a specific activity of ~8500AU/mg. However, the potency of bacteriocin was susceptible to digestion with Proteinase K, Pepsin, SDS, EDTA and Urea. Molecular mass of purified bacteriocin was found to be 1.4kDa using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). The functional group was revealed by FTIR analysis. The cytotoxicity assay (MTT) using purified bacteriocin showed 2 times lower EC50 values compared to SDS. This is the smaller bacteriocin ever reported before from B. coagulans with greater antimicrobial potency with lower cytotoxicity. This bacteriocin raises the possibilities to be used as a biopreservative in food industries.
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U2 - 10.1016/j.ijbiomac.2015.06.005
DO - 10.1016/j.ijbiomac.2015.06.005
M3 - Article
C2 - 26054664
AN - SCOPUS:84930959501
SN - 0141-8130
VL - 79
SP - 800
EP - 806
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -