Abstract
The construction of live recombinant bacterial vaccines requires a reasonably sophisticated genetic system for the introduction, stabilization and expression of foreign antigen genes. Bacteriophages offer a rich collection of tools that can be used for vaccine construction, including site-specific integration-proficient vectors, non-antibiotic selectable markers and signals for efficient transcription and translation of foreign genes. We describe the characterization of a temperate phage of the mycobacteria, mycobacteriophage L5, and application of these phage studies for the construction of recombinant BCG vaccines.
Original language | English (US) |
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Pages (from-to) | 43-47 |
Number of pages | 5 |
Journal | Developments in biological standardization |
Volume | 82 |
State | Published - 1994 |
All Science Journal Classification (ASJC) codes
- General Immunology and Microbiology
- Drug Discovery
- Public Health, Environmental and Occupational Health