Basement membrane: Carbohydrates and X-ray diffraction

P. A. Lee; K. Blasey; I. J. Goldstein; G. B. Pierce

doi:10.1016/0014-4800(69)90061-6

Basement membrane: Carbohydrates and X-ray diffraction

P. A. Lee, K. Blasey, I. J. Goldstein, G. B. Pierce

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16 Scopus citations

Abstract

Epithelial basement membrane, synthesized by a parietal yolk sac carcinoma in vitro or in the ascites, is free of connective tissue cells and products. Carbohydrate components of this basement membrane were d-galactose, d-glucose, mannose, fucose, deoxyribose, ribose, glucosamine, and sialic acid. Total carbohydrate content was 9.50%. Galactosamine, and uronic acid were absent. This composition is compatible with previously reported analyses of basement membrane from non-neoplastic tissues. There was similarity in four out of five intensity maxima with high-angle x-ray diffraction when comparing basement membrane and collagen. A collagen or collagen-like structure may therefore be a basic structural element in epithelial basement membrane.

Original language	English (US)
Pages (from-to)	323-330
Number of pages	8
Journal	Experimental and Molecular Pathology
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/0014-4800(69)90061-6
State	Published - Jun 1969

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Molecular Biology
Clinical Biochemistry

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10.1016/0014-4800(69)90061-6

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title = "Basement membrane: Carbohydrates and X-ray diffraction",

abstract = "Epithelial basement membrane, synthesized by a parietal yolk sac carcinoma in vitro or in the ascites, is free of connective tissue cells and products. Carbohydrate components of this basement membrane were d-galactose, d-glucose, mannose, fucose, deoxyribose, ribose, glucosamine, and sialic acid. Total carbohydrate content was 9.50%. Galactosamine, and uronic acid were absent. This composition is compatible with previously reported analyses of basement membrane from non-neoplastic tissues. There was similarity in four out of five intensity maxima with high-angle x-ray diffraction when comparing basement membrane and collagen. A collagen or collagen-like structure may therefore be a basic structural element in epithelial basement membrane.",

author = "Lee, {P. A.} and K. Blasey and Goldstein, {I. J.} and Pierce, {G. B.}",

note = "Funding Information: {\textquoteright} This work was supported CA-08201 from the U.S.P.H.S. {\textquoteright} Medical Scientist Fellow {\textquoteright} American Cancer Society, University of Colorado Medical",

year = "1969",

month = jun,

doi = "10.1016/0014-4800(69)90061-6",

language = "English (US)",

volume = "10",

pages = "323--330",

journal = "Experimental and Molecular Pathology",

issn = "0014-4800",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Basement membrane

T2 - Carbohydrates and X-ray diffraction

AU - Lee, P. A.

AU - Blasey, K.

AU - Goldstein, I. J.

AU - Pierce, G. B.

N1 - Funding Information: ’ This work was supported CA-08201 from the U.S.P.H.S. ’ Medical Scientist Fellow ’ American Cancer Society, University of Colorado Medical

PY - 1969/6

Y1 - 1969/6

N2 - Epithelial basement membrane, synthesized by a parietal yolk sac carcinoma in vitro or in the ascites, is free of connective tissue cells and products. Carbohydrate components of this basement membrane were d-galactose, d-glucose, mannose, fucose, deoxyribose, ribose, glucosamine, and sialic acid. Total carbohydrate content was 9.50%. Galactosamine, and uronic acid were absent. This composition is compatible with previously reported analyses of basement membrane from non-neoplastic tissues. There was similarity in four out of five intensity maxima with high-angle x-ray diffraction when comparing basement membrane and collagen. A collagen or collagen-like structure may therefore be a basic structural element in epithelial basement membrane.

AB - Epithelial basement membrane, synthesized by a parietal yolk sac carcinoma in vitro or in the ascites, is free of connective tissue cells and products. Carbohydrate components of this basement membrane were d-galactose, d-glucose, mannose, fucose, deoxyribose, ribose, glucosamine, and sialic acid. Total carbohydrate content was 9.50%. Galactosamine, and uronic acid were absent. This composition is compatible with previously reported analyses of basement membrane from non-neoplastic tissues. There was similarity in four out of five intensity maxima with high-angle x-ray diffraction when comparing basement membrane and collagen. A collagen or collagen-like structure may therefore be a basic structural element in epithelial basement membrane.

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Basement membrane: Carbohydrates and X-ray diffraction

Abstract

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