TY - GEN
T1 - Battery-Powered Portable Multiplexed RT - PCR for HIV-1 and HIV-2 Viral Load Testing at LIMIC
AU - Liu, Tianyi
AU - Politza, Anthony J.
AU - Ahamed, Md Ahasan
AU - Kshirsagar, Aneesh
AU - Guan, Weihua
N1 - Publisher Copyright:
© 2024 IEEE.
PY - 2024
Y1 - 2024
N2 - HIV is a major health issue in LMICs, necessitating precise viral load (VL) monitoring for effective management. Antiretroviral therapy (ART) has made HIV manageable, underscoring the need for effective VL testing to achieve the 95-95-95 targets. The diversity of HIV subtypes in LMICs calls for innovative diagnostic solutions. Here, we demonstrate a portable multiplex RT-PCR device designed for fast and cost-effective testing of HIV-1 and HIV-2 using finger-prick blood in LMICs. It features on-site RNA extraction and real-time multiplex RT-PCR targeting HIV-1, HIV-2, and an internal process control of RNase P. The cost per test is approximately $10, based on laboratory-scale material costs. The device offers a sample-to-answer turnaround time of less than 70 minutes. Our internally controlled device exhibited 100% sensitivity and specificity against HIV-1, and 100% sensitivity and 90.91 % specificity against HIV-2.
AB - HIV is a major health issue in LMICs, necessitating precise viral load (VL) monitoring for effective management. Antiretroviral therapy (ART) has made HIV manageable, underscoring the need for effective VL testing to achieve the 95-95-95 targets. The diversity of HIV subtypes in LMICs calls for innovative diagnostic solutions. Here, we demonstrate a portable multiplex RT-PCR device designed for fast and cost-effective testing of HIV-1 and HIV-2 using finger-prick blood in LMICs. It features on-site RNA extraction and real-time multiplex RT-PCR targeting HIV-1, HIV-2, and an internal process control of RNase P. The cost per test is approximately $10, based on laboratory-scale material costs. The device offers a sample-to-answer turnaround time of less than 70 minutes. Our internally controlled device exhibited 100% sensitivity and specificity against HIV-1, and 100% sensitivity and 90.91 % specificity against HIV-2.
UR - https://www.scopus.com/pages/publications/85215274566
UR - https://www.scopus.com/pages/publications/85215274566#tab=citedBy
U2 - 10.1109/SENSORS60989.2024.10785218
DO - 10.1109/SENSORS60989.2024.10785218
M3 - Conference contribution
AN - SCOPUS:85215274566
T3 - Proceedings of IEEE Sensors
BT - 2024 IEEE Sensors, SENSORS 2024 - Conference Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2024 IEEE Sensors, SENSORS 2024
Y2 - 20 October 2024 through 23 October 2024
ER -
