Bed bugs evolved unique adaptive strategy to resist pyrethroid insecticides

Fang Zhu; Hemant Gujar; Jennifer R. Gordon; Kenneth F. Haynes; Michael F. Potter; Subba R. Palli

doi:10.1038/srep01456

Bed bugs evolved unique adaptive strategy to resist pyrethroid insecticides

Fang Zhu, Hemant Gujar, Jennifer R. Gordon, Kenneth F. Haynes, Michael F. Potter, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

Recent advances in genomic and post-genomic technologies have facilitated a genome-wide analysis of the insecticide resistance-associated genes in insects. Through bed bug, Cimex lectularius transcriptome analysis, we identified 14 molecular markers associated with pyrethroid resistance. Our studies revealed that most of the resistance-associated genes functioning in diverse mechanisms are expressed in the epidermal layer of the integument, which could prevent or slow down the toxin from reaching the target sites on nerve cells, where an additional layer of resistance (kdr) is possible. This strategy evolved in bed bugs is based on their unique morphological, physiological and behavioral characteristics and has not been reported in any other insect species. RNA interference-aided knockdown of resistance associated genes showed the relative contribution of each mechanism towards overall resistance development. Understanding the complexity of adaptive strategies employed by bed bugs will help in designing the most effective and sustainable bed bug control methods.

Original language	English (US)
Article number	1456
Journal	Scientific reports
Volume	3
DOIs	https://doi.org/10.1038/srep01456
State	Published - 2013

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title = "Bed bugs evolved unique adaptive strategy to resist pyrethroid insecticides",

abstract = "Recent advances in genomic and post-genomic technologies have facilitated a genome-wide analysis of the insecticide resistance-associated genes in insects. Through bed bug, Cimex lectularius transcriptome analysis, we identified 14 molecular markers associated with pyrethroid resistance. Our studies revealed that most of the resistance-associated genes functioning in diverse mechanisms are expressed in the epidermal layer of the integument, which could prevent or slow down the toxin from reaching the target sites on nerve cells, where an additional layer of resistance (kdr) is possible. This strategy evolved in bed bugs is based on their unique morphological, physiological and behavioral characteristics and has not been reported in any other insect species. RNA interference-aided knockdown of resistance associated genes showed the relative contribution of each mechanism towards overall resistance development. Understanding the complexity of adaptive strategies employed by bed bugs will help in designing the most effective and sustainable bed bug control methods.",

author = "Fang Zhu and Hemant Gujar and Gordon, {Jennifer R.} and Haynes, {Kenneth F.} and Potter, {Michael F.} and Palli, {Subba R.}",

note = "Funding Information: The authors are grateful to Shelby Stamper, Scott Bessin, and Mark Goodman (University of Kentucky) for collecting bed bug samples. We thank Dr. David R. Nelson (University of Tennessee) for help in naming Cimex lectularius P450s. We thank Timothy Walter Moural for his help with language editing. Supported by grants from National Research Initiative of the USDA-NIFA (2011-67013-30143) and Bayer Environmental Science. The paper is contribution (13-08-43) from Kentucky Agricultural Experiment Station.",

year = "2013",

doi = "10.1038/srep01456",

language = "English (US)",

volume = "3",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

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AU - Zhu, Fang

AU - Gujar, Hemant

AU - Gordon, Jennifer R.

AU - Haynes, Kenneth F.

AU - Potter, Michael F.

AU - Palli, Subba R.

N1 - Funding Information: The authors are grateful to Shelby Stamper, Scott Bessin, and Mark Goodman (University of Kentucky) for collecting bed bug samples. We thank Dr. David R. Nelson (University of Tennessee) for help in naming Cimex lectularius P450s. We thank Timothy Walter Moural for his help with language editing. Supported by grants from National Research Initiative of the USDA-NIFA (2011-67013-30143) and Bayer Environmental Science. The paper is contribution (13-08-43) from Kentucky Agricultural Experiment Station.

PY - 2013

Y1 - 2013

N2 - Recent advances in genomic and post-genomic technologies have facilitated a genome-wide analysis of the insecticide resistance-associated genes in insects. Through bed bug, Cimex lectularius transcriptome analysis, we identified 14 molecular markers associated with pyrethroid resistance. Our studies revealed that most of the resistance-associated genes functioning in diverse mechanisms are expressed in the epidermal layer of the integument, which could prevent or slow down the toxin from reaching the target sites on nerve cells, where an additional layer of resistance (kdr) is possible. This strategy evolved in bed bugs is based on their unique morphological, physiological and behavioral characteristics and has not been reported in any other insect species. RNA interference-aided knockdown of resistance associated genes showed the relative contribution of each mechanism towards overall resistance development. Understanding the complexity of adaptive strategies employed by bed bugs will help in designing the most effective and sustainable bed bug control methods.

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Bed bugs evolved unique adaptive strategy to resist pyrethroid insecticides

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