TY - JOUR
T1 - Bile acids conjugation in human bile is not random
T2 - New insights from 1H-NMR spectroscopy at 800 MHz
AU - Nagana Gowda, G. A.
AU - Shanaiah, Narasimhamurthy
AU - Cooper, Amanda
AU - Maluccio, Mary
AU - Raftery, Daniel
N1 - Funding Information:
Acknowledgments This work was supported by the Walther Cancer Institute Multi-Institution Cancer Research Seed Project, the Purdue Oncological and Cancer Centers, and a collaborative research grant
PY - 2009/6
Y1 - 2009/6
N2 - Bile acids constitute a group of structurally closely related molecules and represent the most abundant constituents of human bile. Investigations of bile acids have garnered increased interest owing to their recently discovered additional biological functions including their role as signaling molecules that govern glucose, fat and energy metabolism. Recent NMR methodological developments have enabled single-step analysis of several highly abundant and common glycine- and taurine- conjugated bile acids, such as glycocholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, taurocholic acid, taurodeoxycholic acid, and taurochenodeoxycholic acid. Investigation of these conjugated bile acids in human bile employing high field (800 MHz) 1H-NMR spectroscopy reveals that the ratios between two glycine-conjugated bile acids and their taurine counterparts correlate positively (R 2 = 0.83-0.97; p = 0.001 × 10-2-0.006 × 10-7) as do the ratios between a glycine-conjugated bile acid and its taurine counterpart (R 2 = 0.92-0.95; p = 0.004 × 10-3-0.002 × 10-10). Using such correlations, concentration of individual bile acids in each sample could be predicted in good agreement with the experimentally determined values. These insights into the pattern of bile acid conjugation in human bile between glycine and taurine promise useful clues to the mechanism of bile acids' biosynthesis, conjugation and enterohepatic circulation, and may improve our understanding of the role of individual conjugated bile acids in health and disease.
AB - Bile acids constitute a group of structurally closely related molecules and represent the most abundant constituents of human bile. Investigations of bile acids have garnered increased interest owing to their recently discovered additional biological functions including their role as signaling molecules that govern glucose, fat and energy metabolism. Recent NMR methodological developments have enabled single-step analysis of several highly abundant and common glycine- and taurine- conjugated bile acids, such as glycocholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, taurocholic acid, taurodeoxycholic acid, and taurochenodeoxycholic acid. Investigation of these conjugated bile acids in human bile employing high field (800 MHz) 1H-NMR spectroscopy reveals that the ratios between two glycine-conjugated bile acids and their taurine counterparts correlate positively (R 2 = 0.83-0.97; p = 0.001 × 10-2-0.006 × 10-7) as do the ratios between a glycine-conjugated bile acid and its taurine counterpart (R 2 = 0.92-0.95; p = 0.004 × 10-3-0.002 × 10-10). Using such correlations, concentration of individual bile acids in each sample could be predicted in good agreement with the experimentally determined values. These insights into the pattern of bile acid conjugation in human bile between glycine and taurine promise useful clues to the mechanism of bile acids' biosynthesis, conjugation and enterohepatic circulation, and may improve our understanding of the role of individual conjugated bile acids in health and disease.
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U2 - 10.1007/s11745-009-3296-4
DO - 10.1007/s11745-009-3296-4
M3 - Article
C2 - 19373503
AN - SCOPUS:67349119500
SN - 0024-4201
VL - 44
SP - 527
EP - 535
JO - Lipids
JF - Lipids
IS - 6
ER -