TY - JOUR
T1 - Biliary bile acid profiles of domestic fowl as determined by high performance liquid chromatography and fast atom bombardment mass spectrometry
AU - Elkin, R. G.
AU - Wood, K. V.
AU - Hagey, L. R.
N1 - Funding Information:
The major bile acid of domestic fowl is chenodeoxycholyltaurine \[CDCT\]( Hill, 1983). Other bile acids reported to be present in avian bile include cholyltaurine \[CT\] (Haslewood, 1971; Duke, 1986), allocholyltaurine \[ACT\] (Haslewood, 1971; Duke, 1986), phocaecholyltaurine \[PCT\]( Klinot et al., 1986; Jirsa et al., 1989) and lithocholyltaurine \[LCT\] (Serafin and Nesheim, 1970; Crompton and Nesheim, 1970; Jirsa et al., 1989). In much of the previous work on avian biliary bile acids, these compounds were isolated, chromatographed (paper, thin layer, or gas chromatography), and identified by Rr values or retention times. In certain instances, compound identities were confirmed by infrared spectroscopy (Anderson and Haslewood, 1962). However, an *Journal paper number 12226, Purdue University Agricul-tural Experiment Station. Presented in part at the Second International Symposium on Mass Spectrometry in the Health and Life Sciences, San Franciso, CA, 27 31 August, 1989. The work of L. R. Hagey was supported in part by grants DK-21506 and DK-32130 from the National Institutes of Health.
PY - 1990
Y1 - 1990
N2 - 1. 1. The biliary bile acid profiles of domestic chickens (Gallus domesticus), turkeys (Meleagris gallopavo), and ducks (Anas platyrhynchos) were determiend by high performance liquid chromatography (HPLC) and fast atom bombardment mass spectrometry (FAB-MS). 2. 2. Chenodeoxycholyltaurine and cholyltaurine were the predominant bile acids in chicken and turkey bile, whereas duck bile contained primarily chenodeoxycholyltaurine and phocaecholyltaurine. 3. 3. Allocholyltaurine was also detected in chicken and turkey bile, but not in duck bile. 4. 4. FAB-MS analyses of individual HPLC peak fractions from chicken and duck bile extracts confirmed the presence of either taurine-conjugated dihydroxy- or trihydroxycholanoates. 5. 5. Direct FAB-MS analyses of avian bile extracts not subjected to HPLC permitted a rapid assessment of the relative proportion of taurine-conjugated dihydroxy- to trihydroxycholanoates.
AB - 1. 1. The biliary bile acid profiles of domestic chickens (Gallus domesticus), turkeys (Meleagris gallopavo), and ducks (Anas platyrhynchos) were determiend by high performance liquid chromatography (HPLC) and fast atom bombardment mass spectrometry (FAB-MS). 2. 2. Chenodeoxycholyltaurine and cholyltaurine were the predominant bile acids in chicken and turkey bile, whereas duck bile contained primarily chenodeoxycholyltaurine and phocaecholyltaurine. 3. 3. Allocholyltaurine was also detected in chicken and turkey bile, but not in duck bile. 4. 4. FAB-MS analyses of individual HPLC peak fractions from chicken and duck bile extracts confirmed the presence of either taurine-conjugated dihydroxy- or trihydroxycholanoates. 5. 5. Direct FAB-MS analyses of avian bile extracts not subjected to HPLC permitted a rapid assessment of the relative proportion of taurine-conjugated dihydroxy- to trihydroxycholanoates.
UR - https://www.scopus.com/pages/publications/0025217374
UR - https://www.scopus.com/pages/publications/0025217374#tab=citedBy
U2 - 10.1016/0305-0491(90)90356-X
DO - 10.1016/0305-0491(90)90356-X
M3 - Article
C2 - 2364668
AN - SCOPUS:0025217374
SN - 0305-0491
VL - 96
SP - 157
EP - 161
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
IS - 1
ER -