TY - JOUR
T1 - Binding sites for [3H]-acetylcholine and 125I-α-bungarotoxin in the optic ganglion of Loligo pealii
AU - Chen, Shu-jen
AU - Spathis, Rita
AU - Schmidt, Jakob
N1 - Funding Information:
*This research was supported in part by NIH grant NS18839 and USAMRAA contract DAMD17-86-C-6058. tThe abbreviations used are: AcCh, acetylcholine; AcChR, acetylcholine receptor; aBuTx, a-bungarotoxin.
PY - 1988
Y1 - 1988
N2 - 1. 1. In the optic ganglion of Loligo pealii, binding sites for [3H]-acetylcholine (KD: 5.2 × 10-7 M; Bmax: 1.7 × 10-11 mol/g tissue) and 125I-α-bungarotoxin (KD: 3.3 × 10-9 M; Bmax: 9.7 × 10-11 mol/g tissue) were observed. 2. 2. Both sites are blocked by nicotinic compounds, but differ significantly in their affinity for individual ligands, with the acetylcholine site preferentially binding agonists, and the toxin site, antagonists. 3. 3. The acetylcholine site is substantially more thermolabile than the toxin site. 4. 4. A partial separation of the two binding activities is accomplished by sucrose density centrifugation. 5. 5. These observations and a comparison with other tissues (Torpedo californica electroplaque; chick optic lobe; rat brain) suggest the presence, in the squid, of more than one kind of neuronal nicotinic receptor.
AB - 1. 1. In the optic ganglion of Loligo pealii, binding sites for [3H]-acetylcholine (KD: 5.2 × 10-7 M; Bmax: 1.7 × 10-11 mol/g tissue) and 125I-α-bungarotoxin (KD: 3.3 × 10-9 M; Bmax: 9.7 × 10-11 mol/g tissue) were observed. 2. 2. Both sites are blocked by nicotinic compounds, but differ significantly in their affinity for individual ligands, with the acetylcholine site preferentially binding agonists, and the toxin site, antagonists. 3. 3. The acetylcholine site is substantially more thermolabile than the toxin site. 4. 4. A partial separation of the two binding activities is accomplished by sucrose density centrifugation. 5. 5. These observations and a comparison with other tissues (Torpedo californica electroplaque; chick optic lobe; rat brain) suggest the presence, in the squid, of more than one kind of neuronal nicotinic receptor.
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U2 - 10.1016/0742-8413(88)90005-9
DO - 10.1016/0742-8413(88)90005-9
M3 - Article
C2 - 2902992
AN - SCOPUS:0023887710
SN - 0306-4492
VL - 90
SP - 317
EP - 323
JO - Comparative Biochemistry and Physiology. Part C, Comparative
JF - Comparative Biochemistry and Physiology. Part C, Comparative
IS - 2
ER -