Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle

David A. Castilla-Casadiego, Katherine A. Miranda-Muñõz, Jesse L. Roberts, Anne D. Crowell, David Gonzalez-Nino, Dipankar Choudhury, Frank O. Aparicio-Solis, Shannon L. Servoss, Adrianne M. Rosales, Gary Prinz, Min Zou, Yuntao Zhang, Johann F. Coetzee, Lauren F. Greenlee, Jeremy Powell, Jorge Almodovar

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin. In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested. The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated. In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis. In-vivo studies were performed to analyze the dissolution capability of the microneedle patch. Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches. Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches. In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days. A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days. No abnormal tissue, swelling, or inflammation was observed in the implanted area.

Original languageEnglish (US)
Article numbere0272169
JournalPloS one
Volume17
Issue number8 August
DOIs
StatePublished - Aug 2022

All Science Journal Classification (ASJC) codes

  • General

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