TY - JOUR
T1 - Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically engineered Escherichia coli strain
AU - Yasin, Nazlina Haiza Mohd
AU - Fukuzaki, Masaharu
AU - Maeda, Toshinari
AU - Miyazaki, Toshiki
AU - Maail, Che Mohd Hakiman Che
AU - Ariffin, Hidayah
AU - Wood, Thomas K.
N1 - Funding Information:
The authors would like to acknowledge M.A Hassan from Universiti Putra Malaysia (UPM) and M.A.K.M Zahari from Universiti Malaysia Pahang (UMP) for providing the OPF juice and the Japan Students Services Organization for the scholarship of N.H. Mohd Yasin during this study. This research was supported by the Research Center for Advanced Eco-fitting Technology of Kyushu Institute of Technology and Sasakawa Scientific Research Grant , The Japan Science Society (25-457).
PY - 2013/8/21
Y1 - 2013/8/21
N2 - Biohydrogen is considered a promising and environmentally friendly energy source. Escherichia coli BW25113 hyaB hybC hycA fdoG frdc ldhA aceE has been previously engineered for elevated biohydrogen production from glucose. In this study, we show that this strain can also use biomass from oil palm frond (OPF) juice and sewage sludge as substrates. Substrate improvement was accomplished when hydrogen productivity increased 8-fold after enzymatic treatment of the sludge with a mixture of amylase and cellulase. The OPF juice with sewage sludge provided an optimum carbon/nitrogen ratio since the yield of biohydrogen increased to 1.5 from 1.3 mol H2/mol glucose compared to our previous study. In this study, we also reveal that our engineered strain improved 200-fold biohydrogen productivity from biomass sources compared to the unmodified host. In conclusion, we determined that our engineered strain can use biomass as an alternative substrate for enhanced biohydrogen production.
AB - Biohydrogen is considered a promising and environmentally friendly energy source. Escherichia coli BW25113 hyaB hybC hycA fdoG frdc ldhA aceE has been previously engineered for elevated biohydrogen production from glucose. In this study, we show that this strain can also use biomass from oil palm frond (OPF) juice and sewage sludge as substrates. Substrate improvement was accomplished when hydrogen productivity increased 8-fold after enzymatic treatment of the sludge with a mixture of amylase and cellulase. The OPF juice with sewage sludge provided an optimum carbon/nitrogen ratio since the yield of biohydrogen increased to 1.5 from 1.3 mol H2/mol glucose compared to our previous study. In this study, we also reveal that our engineered strain improved 200-fold biohydrogen productivity from biomass sources compared to the unmodified host. In conclusion, we determined that our engineered strain can use biomass as an alternative substrate for enhanced biohydrogen production.
UR - http://www.scopus.com/inward/record.url?scp=84883132656&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883132656&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2013.06.065
DO - 10.1016/j.ijhydene.2013.06.065
M3 - Article
AN - SCOPUS:84883132656
SN - 0360-3199
VL - 38
SP - 10277
EP - 10283
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 25
ER -