Biological synthesis of cationic gold nanoparticles and binding of plasmid DNA

D. Raju; Rishi K. Vishwakarma; Bashir M. Khan; Urmil J. Mehta; Absar Ahmad

doi:10.1016/j.matlet.2014.05.021

Biological synthesis of cationic gold nanoparticles and binding of plasmid DNA

D. Raju, Rishi K. Vishwakarma, Bashir M. Khan, Urmil J. Mehta, Absar Ahmad

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Nanobiotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials. The present work focuses on synthesis of cationic gold nanoparticles (C-GNPs) for biological applications, especially in gene and drug delivery studies. A biosynthesis methodology has been developed for the functionalization of gold nanoparticles to cationic nature. The synthesis of C-GNPs was done by using peanut leaf extract in the presence of cysteamine. The formed C-GNPs were characterized by using UV-visible spectroscopy (UV-vis), the particles sizes and shapes were confirmed by a Transmission electron microscope (TEM) and crystallinity of C-GNPs was characterized by diffraction. The binding of plasmid DNA on the C-GNPs was confirmed by agarose gel electrophoresis.

Original language	English (US)
Pages (from-to)	159-161
Number of pages	3
Journal	Materials Letters
Volume	129
DOIs	https://doi.org/10.1016/j.matlet.2014.05.021
State	Published - Aug 15 2014

All Science Journal Classification (ASJC) codes

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1016/j.matlet.2014.05.021

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@article{921cee19059040e58f2dbd7866f4e98f,

title = "Biological synthesis of cationic gold nanoparticles and binding of plasmid DNA",

abstract = "Nanobiotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials. The present work focuses on synthesis of cationic gold nanoparticles (C-GNPs) for biological applications, especially in gene and drug delivery studies. A biosynthesis methodology has been developed for the functionalization of gold nanoparticles to cationic nature. The synthesis of C-GNPs was done by using peanut leaf extract in the presence of cysteamine. The formed C-GNPs were characterized by using UV-visible spectroscopy (UV-vis), the particles sizes and shapes were confirmed by a Transmission electron microscope (TEM) and crystallinity of C-GNPs was characterized by diffraction. The binding of plasmid DNA on the C-GNPs was confirmed by agarose gel electrophoresis.",

author = "D. Raju and Vishwakarma, {Rishi K.} and Khan, {Bashir M.} and Mehta, {Urmil J.} and Absar Ahmad",

note = "Funding Information: D.R. thanks the Council of Scientific and Industrial Research (CSIR), New Delhi (31/011/(432)/2008-EMR-I) for Senior Research Fellowship. A.A. thanks the Department of Biotechnology, Govt. of India (New Delhi) for the Tata Innovation Fellowship award and financial support through BSC 0112 CSIR. The authors also thank the Center for Material Characterization, CSIR-NCL for TEM analysis. ",

year = "2014",

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doi = "10.1016/j.matlet.2014.05.021",

language = "English (US)",

volume = "129",

pages = "159--161",

journal = "Materials Letters",

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publisher = "Elsevier B.V.",

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T1 - Biological synthesis of cationic gold nanoparticles and binding of plasmid DNA

AU - Raju, D.

AU - Vishwakarma, Rishi K.

AU - Khan, Bashir M.

AU - Mehta, Urmil J.

AU - Ahmad, Absar

N1 - Funding Information: D.R. thanks the Council of Scientific and Industrial Research (CSIR), New Delhi (31/011/(432)/2008-EMR-I) for Senior Research Fellowship. A.A. thanks the Department of Biotechnology, Govt. of India (New Delhi) for the Tata Innovation Fellowship award and financial support through BSC 0112 CSIR. The authors also thank the Center for Material Characterization, CSIR-NCL for TEM analysis.

PY - 2014/8/15

Y1 - 2014/8/15

N2 - Nanobiotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials. The present work focuses on synthesis of cationic gold nanoparticles (C-GNPs) for biological applications, especially in gene and drug delivery studies. A biosynthesis methodology has been developed for the functionalization of gold nanoparticles to cationic nature. The synthesis of C-GNPs was done by using peanut leaf extract in the presence of cysteamine. The formed C-GNPs were characterized by using UV-visible spectroscopy (UV-vis), the particles sizes and shapes were confirmed by a Transmission electron microscope (TEM) and crystallinity of C-GNPs was characterized by diffraction. The binding of plasmid DNA on the C-GNPs was confirmed by agarose gel electrophoresis.

AB - Nanobiotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials. The present work focuses on synthesis of cationic gold nanoparticles (C-GNPs) for biological applications, especially in gene and drug delivery studies. A biosynthesis methodology has been developed for the functionalization of gold nanoparticles to cationic nature. The synthesis of C-GNPs was done by using peanut leaf extract in the presence of cysteamine. The formed C-GNPs were characterized by using UV-visible spectroscopy (UV-vis), the particles sizes and shapes were confirmed by a Transmission electron microscope (TEM) and crystallinity of C-GNPs was characterized by diffraction. The binding of plasmid DNA on the C-GNPs was confirmed by agarose gel electrophoresis.

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DO - 10.1016/j.matlet.2014.05.021

M3 - Article

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VL - 129

SP - 159

EP - 161

JO - Materials Letters

JF - Materials Letters

ER -

Biological synthesis of cationic gold nanoparticles and binding of plasmid DNA

Abstract

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