Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins

Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, Daniel M. Durachko

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.

Original languageEnglish (US)
Pages (from-to)79-88
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2023

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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