Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins

Daniel J. Cosgrove; Nathan K. Hepler; Edward R. Wagner; Daniel M. Durachko

doi:10.1007/978-1-0716-3151-5_5

Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins

Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, Daniel M. Durachko

Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.

Original language	English (US)
Pages (from-to)	79-88
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2657
DOIs	https://doi.org/10.1007/978-1-0716-3151-5_5
State	Published - 2023

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1007/978-1-0716-3151-5_5

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abstract = "Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.",

author = "Cosgrove, {Daniel J.} and Hepler, {Nathan K.} and Wagner, {Edward R.} and Durachko, {Daniel M.}",

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AU - Wagner, Edward R.

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PY - 2023

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AB - Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.

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Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins

Abstract

All Science Journal Classification (ASJC) codes

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