Biomimetic Bacterial Capsule for Enhanced Aptamer Display and Cell Recognition

Xuelin Wang, Bei Jia, Kyungsene Lee, Brandon Davis, Connie Wen, Yixun Wang, Hong Zheng, Yong Wang

Research output: Contribution to journalArticlepeer-review

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Great effort has been made to encapsulate or coat living mammalian cells for a variety of applications ranging from diabetes treatment to three-dimensional printing. However, no study has reported the synthesis of a biomimetic bacterial capsule to display high-affinity aptamers on the cell surface for enhanced cell recognition. Therefore, we synthesized an ultrathin alginate-polylysine coating to display aptamers on the surface of living cells with natural killer (NK) cells as a model. The results show that this coating-mediated aptamer display is more stable than direct cholesterol insertion into the lipid bilayer. The half-life of the aptamer on the cell surface can be increased from less than 1.5 to over 20 h. NK cells coated with the biomimetic bacterial capsule exhibit a high efficiency in recognizing and killing target cells. Therefore, this work has demonstrated a promising cell coating method for the display of aptamers for enhanced cell recognition.

Original languageEnglish (US)
Pages (from-to)868-877
Number of pages10
JournalJournal of the American Chemical Society
Issue number1
StatePublished - Jan 10 2024

All Science Journal Classification (ASJC) codes

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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