TY - JOUR
T1 - Biosynthesis of 1-octen-3-ol and 10-oxo-trans-8-decenoic acid using a crude homogenate of Agaricus bisporus
T2 - Optimization of the reaction: Kinetic factors
AU - Morawicki, Ruben O.
AU - Beelman, Robert B.
AU - Peterson, Devin
AU - Ziegler, Gregory
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/1
Y1 - 2005/1
N2 - 1-Octen-3-ol and 10-oxo-trans-8-decenoic acid (ODA) are the products of the enzymic breakdown of linoleic acid (LA) in mushrooms by a lipoxygenase and a hydroperoxide lyase. Based on this reaction, natural 1-octen-3-ol and ODA can be produced with a crude enzyme homogenate from mushrooms in a bioreactor. The influence on this reaction of temperature (5-24°C), concentration of LA (1.3-13.3 mg/g mushrooms), stirring speed (500-1500 rpm), concentration of oxygen (23.2-100% saturation), and pH (6.5-7.5) was studied in a 1 l standard bioreactor by monitoring the production of 1-octen-3-ol. A central composite rotatable and an algorithmic experimental design were used to study the five factors. Only LA concentration and temperature were found to affect significantly the yield of the reaction. The optimal parameters that maximized the production of 1-octen-3-ol were 12 mg of linoleic acid (99% purity) per gram of mushroom, 7.8°C, and a pH of 7.5; with a predicted yield of 2.7±0.4 mg of 1-octen-3-ol per gram of mushroom.
AB - 1-Octen-3-ol and 10-oxo-trans-8-decenoic acid (ODA) are the products of the enzymic breakdown of linoleic acid (LA) in mushrooms by a lipoxygenase and a hydroperoxide lyase. Based on this reaction, natural 1-octen-3-ol and ODA can be produced with a crude enzyme homogenate from mushrooms in a bioreactor. The influence on this reaction of temperature (5-24°C), concentration of LA (1.3-13.3 mg/g mushrooms), stirring speed (500-1500 rpm), concentration of oxygen (23.2-100% saturation), and pH (6.5-7.5) was studied in a 1 l standard bioreactor by monitoring the production of 1-octen-3-ol. A central composite rotatable and an algorithmic experimental design were used to study the five factors. Only LA concentration and temperature were found to affect significantly the yield of the reaction. The optimal parameters that maximized the production of 1-octen-3-ol were 12 mg of linoleic acid (99% purity) per gram of mushroom, 7.8°C, and a pH of 7.5; with a predicted yield of 2.7±0.4 mg of 1-octen-3-ol per gram of mushroom.
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U2 - 10.1016/j.procbio.2003.11.037
DO - 10.1016/j.procbio.2003.11.037
M3 - Article
AN - SCOPUS:4444354031
SN - 0032-9592
VL - 40
SP - 131
EP - 137
JO - Process Biochemistry
JF - Process Biochemistry
IS - 1
ER -