Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila

Yongfei Yang; Vikas Gupta; Li Lun Ho; Bo Zhou; Qichang Fan; Zuoyan Zhu; Wenxia Zhang; Zhi Chun Lai

doi:10.1016/j.febslet.2008.04.050

Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila

Yongfei Yang, Vikas Gupta, Li Lun Ho, Bo Zhou, Qichang Fan, Zuoyan Zhu, Wenxia Zhang, Zhi Chun Lai

Research output: Contribution to journal › Article › peer-review

Abstract

The Drosophila mats gene plays a critical role in growth control. Using molecular genetic approaches we investigated how mats is regulated in development. A 2236-bp genomic sequence that contains entire mats including upstream and downstream intergenic regions can rescue mats mutant phenotypes, indicating that regulatory elements necessary for proper mats expression are mostly retained. However, constructs without the upstream or downstream intergenic region failed to rescue mats mutants, demonstrating the functional importance of these sequences. Moreover, mats expression is reduced in mats^e17, a mats allele with over one-third of the downstream intergenic region deleted. Consistent with a model that the downstream intergenic region is critical for mats activity, this sequence contains evolutionarily conserved elements and has enhancer activities.

Original language	English (US)
Pages (from-to)	1766-1770
Number of pages	5
Journal	FEBS Letters
Volume	582
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2008.04.050
State	Published - May 28 2008

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2008.04.050

Cite this

@article{63eb278c8ee24c17a420676f66bae380,

title = "Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila",

abstract = "The Drosophila mats gene plays a critical role in growth control. Using molecular genetic approaches we investigated how mats is regulated in development. A 2236-bp genomic sequence that contains entire mats including upstream and downstream intergenic regions can rescue mats mutant phenotypes, indicating that regulatory elements necessary for proper mats expression are mostly retained. However, constructs without the upstream or downstream intergenic region failed to rescue mats mutants, demonstrating the functional importance of these sequences. Moreover, mats expression is reduced in matse17, a mats allele with over one-third of the downstream intergenic region deleted. Consistent with a model that the downstream intergenic region is critical for mats activity, this sequence contains evolutionarily conserved elements and has enhancer activities.",

author = "Yongfei Yang and Vikas Gupta and Ho, {Li Lun} and Bo Zhou and Qichang Fan and Zuoyan Zhu and Wenxia Zhang and Lai, {Zhi Chun}",

note = "Funding Information: We would like to thank Margaret Rohrbaugh for generating mats e17 , Takeshi Shimizu for teaching V.G. molecular and genetic techniques, and Xiaomu Wei for teaching Y.Y. the fly transformation procedure and for help generating four additional P[mats-2236] lines. We also thank Kai Cui, Erin Merkel, Xinhua Lin, Lixia Wang, Zhaohui Wang and Chengying Yang for their help in this project. Confocal imaging was done at the Center for Quantitative Cell Analysis at the Huck Institutes of the Life Sciences, Pennsylvania State University. This work was supported by Grants from the National Natural Science Foundation of China (Grant No. 30510403188) and NSF.",

year = "2008",

month = may,

day = "28",

doi = "10.1016/j.febslet.2008.04.050",

language = "English (US)",

volume = "582",

pages = "1766--1770",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "12",

}

TY - JOUR

T1 - Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila

AU - Yang, Yongfei

AU - Gupta, Vikas

AU - Ho, Li Lun

AU - Zhou, Bo

AU - Fan, Qichang

AU - Zhu, Zuoyan

AU - Zhang, Wenxia

AU - Lai, Zhi Chun

N1 - Funding Information: We would like to thank Margaret Rohrbaugh for generating mats e17 , Takeshi Shimizu for teaching V.G. molecular and genetic techniques, and Xiaomu Wei for teaching Y.Y. the fly transformation procedure and for help generating four additional P[mats-2236] lines. We also thank Kai Cui, Erin Merkel, Xinhua Lin, Lixia Wang, Zhaohui Wang and Chengying Yang for their help in this project. Confocal imaging was done at the Center for Quantitative Cell Analysis at the Huck Institutes of the Life Sciences, Pennsylvania State University. This work was supported by Grants from the National Natural Science Foundation of China (Grant No. 30510403188) and NSF.

PY - 2008/5/28

Y1 - 2008/5/28

N2 - The Drosophila mats gene plays a critical role in growth control. Using molecular genetic approaches we investigated how mats is regulated in development. A 2236-bp genomic sequence that contains entire mats including upstream and downstream intergenic regions can rescue mats mutant phenotypes, indicating that regulatory elements necessary for proper mats expression are mostly retained. However, constructs without the upstream or downstream intergenic region failed to rescue mats mutants, demonstrating the functional importance of these sequences. Moreover, mats expression is reduced in matse17, a mats allele with over one-third of the downstream intergenic region deleted. Consistent with a model that the downstream intergenic region is critical for mats activity, this sequence contains evolutionarily conserved elements and has enhancer activities.

AB - The Drosophila mats gene plays a critical role in growth control. Using molecular genetic approaches we investigated how mats is regulated in development. A 2236-bp genomic sequence that contains entire mats including upstream and downstream intergenic regions can rescue mats mutant phenotypes, indicating that regulatory elements necessary for proper mats expression are mostly retained. However, constructs without the upstream or downstream intergenic region failed to rescue mats mutants, demonstrating the functional importance of these sequences. Moreover, mats expression is reduced in matse17, a mats allele with over one-third of the downstream intergenic region deleted. Consistent with a model that the downstream intergenic region is critical for mats activity, this sequence contains evolutionarily conserved elements and has enhancer activities.

UR - http://www.scopus.com/inward/record.url?scp=43549103277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43549103277&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.04.050

DO - 10.1016/j.febslet.2008.04.050

M3 - Article

C2 - 18472003

AN - SCOPUS:43549103277

SN - 0014-5793

VL - 582

SP - 1766

EP - 1770

JO - FEBS Letters

JF - FEBS Letters

IS - 12

ER -

Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this