TY - JOUR
T1 - Bovine luteal cells elicit major histocompatibility complex class II- dependent T-cell proliferation
AU - Petroff, Margaret Greene
AU - Coggeshall, K. Mark
AU - Jones, Leslie S.
AU - Pate, Joy L.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/10
Y1 - 1997/10
N2 - Major histocompatibility complex (MHC) class II molecules are expressed in the bovine corpus luteum (CL) in a manner correlating with luteolysis. Whether bovine luteal cells can stimulate T-cell proliferation in a class II- restricted manner was investigated. Staphylococcal enterotoxin B (SEB) enhances T-cell proliferation by a mechanism requiring MHC class II molecules and was used to examine stimulation of T-cell proliferation by luteal cells. Luteal cells from midcycle or regressing CL (induced by prostaglandin F(2α)) were cocultured with autologous T cells in the presence of no treatment, SEB (1 μg/ml), or SEB + anti-MHC class II antibody (3 μg/ml); and proliferation was assessed by incorporation of tritiated thymidine. T cells proliferated in the presence of cells from regressing CL more than when in the presence of midcycle cells (118 309 ± 20.567 vs. 75 261 ± 12 494 cpm; p < 0.05). Anti- MHC attenuated this response of cells from regressing CL (81 108 cpm ± 13 249; p < 0.05). Without SEB, T cells proliferated when cultured with cells from regressing, but not midcycle, CL (4637 ± 816 vs. 2117 ± 589 cpm; p < 0.03). These results suggest that luteal cells can function as antigen- presenting cells in vitro and that prostaglandin F(2α) may enhance their ability to present antigen.
AB - Major histocompatibility complex (MHC) class II molecules are expressed in the bovine corpus luteum (CL) in a manner correlating with luteolysis. Whether bovine luteal cells can stimulate T-cell proliferation in a class II- restricted manner was investigated. Staphylococcal enterotoxin B (SEB) enhances T-cell proliferation by a mechanism requiring MHC class II molecules and was used to examine stimulation of T-cell proliferation by luteal cells. Luteal cells from midcycle or regressing CL (induced by prostaglandin F(2α)) were cocultured with autologous T cells in the presence of no treatment, SEB (1 μg/ml), or SEB + anti-MHC class II antibody (3 μg/ml); and proliferation was assessed by incorporation of tritiated thymidine. T cells proliferated in the presence of cells from regressing CL more than when in the presence of midcycle cells (118 309 ± 20.567 vs. 75 261 ± 12 494 cpm; p < 0.05). Anti- MHC attenuated this response of cells from regressing CL (81 108 cpm ± 13 249; p < 0.05). Without SEB, T cells proliferated when cultured with cells from regressing, but not midcycle, CL (4637 ± 816 vs. 2117 ± 589 cpm; p < 0.03). These results suggest that luteal cells can function as antigen- presenting cells in vitro and that prostaglandin F(2α) may enhance their ability to present antigen.
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U2 - 10.1095/biolreprod57.4.887
DO - 10.1095/biolreprod57.4.887
M3 - Article
C2 - 9314594
AN - SCOPUS:0030954827
SN - 0006-3363
VL - 57
SP - 887
EP - 893
JO - Biology of reproduction
JF - Biology of reproduction
IS - 4
ER -