Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors

S. P. Perrine; B. A. Miller; M. F. Greene; R. A. Cohen; N. Cook; C. Shackleton; D. V. Faller

doi:10.1016/0006-291X(87)90932-6

Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors

S. P. Perrine
, B. A. Miller
, M. F. Greene
, R. A. Cohen
, N. Cook
, C. Shackleton
, D. V. Faller

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The γ→β globin gene switch in humans is normally on a set developmental clock but is delayed in infants of diabetic mothers. We cultured cord blood erythroid progenitors and assayed globin produced in the presence and absence of metabolites that are elevated in such infants. Analogues of butyric acid at supranormal concentrations significantly augmented γ and inhibited β globin expression. The uptake of α-amino-n-butyric acid into colony-derived erythroblasts was increased in the presence of supranormal insulin. These findings suggest that elevated levels of α-amino-n-butyric acid and insulin in the developing fetus delay the globin switch and may offer potential for augmenting γ globin expression in the β globin chain diseases.

Original language	English (US)
Pages (from-to)	694-700
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	148
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)90932-6
State	Published - Oct 29 1987

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(87)90932-6

Cite this

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title = "Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors",

abstract = "The γ→β globin gene switch in humans is normally on a set developmental clock but is delayed in infants of diabetic mothers. We cultured cord blood erythroid progenitors and assayed globin produced in the presence and absence of metabolites that are elevated in such infants. Analogues of butyric acid at supranormal concentrations significantly augmented γ and inhibited β globin expression. The uptake of α-amino-n-butyric acid into colony-derived erythroblasts was increased in the presence of supranormal insulin. These findings suggest that elevated levels of α-amino-n-butyric acid and insulin in the developing fetus delay the globin switch and may offer potential for augmenting γ globin expression in the β globin chain diseases.",

author = "Perrine, \{S. P.\} and Miller, \{B. A.\} and Greene, \{M. F.\} and Cohen, \{R. A.\} and N. Cook and C. Shackleton and Faller, \{D. V.\}",

note = "Funding Information: by Grant HL-37118 from the National Heart, Lung, and Blood and by a grant from the Juvenile Diabetes Foundation Dr. Miller is the recipient of a Clinical Investigator Natlonal Heart, Lung, and Blood Institute. Dr. Faller 1s of a Senior Faculty Award from the American Cancer Society.",

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doi = "10.1016/0006-291X(87)90932-6",

language = "English (US)",

volume = "148",

pages = "694--700",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors

AU - Perrine, S. P.

AU - Miller, B. A.

AU - Greene, M. F.

AU - Cohen, R. A.

AU - Cook, N.

AU - Shackleton, C.

AU - Faller, D. V.

N1 - Funding Information: by Grant HL-37118 from the National Heart, Lung, and Blood and by a grant from the Juvenile Diabetes Foundation Dr. Miller is the recipient of a Clinical Investigator Natlonal Heart, Lung, and Blood Institute. Dr. Faller 1s of a Senior Faculty Award from the American Cancer Society.

PY - 1987/10/29

Y1 - 1987/10/29

N2 - The γ→β globin gene switch in humans is normally on a set developmental clock but is delayed in infants of diabetic mothers. We cultured cord blood erythroid progenitors and assayed globin produced in the presence and absence of metabolites that are elevated in such infants. Analogues of butyric acid at supranormal concentrations significantly augmented γ and inhibited β globin expression. The uptake of α-amino-n-butyric acid into colony-derived erythroblasts was increased in the presence of supranormal insulin. These findings suggest that elevated levels of α-amino-n-butyric acid and insulin in the developing fetus delay the globin switch and may offer potential for augmenting γ globin expression in the β globin chain diseases.

AB - The γ→β globin gene switch in humans is normally on a set developmental clock but is delayed in infants of diabetic mothers. We cultured cord blood erythroid progenitors and assayed globin produced in the presence and absence of metabolites that are elevated in such infants. Analogues of butyric acid at supranormal concentrations significantly augmented γ and inhibited β globin expression. The uptake of α-amino-n-butyric acid into colony-derived erythroblasts was increased in the presence of supranormal insulin. These findings suggest that elevated levels of α-amino-n-butyric acid and insulin in the developing fetus delay the globin switch and may offer potential for augmenting γ globin expression in the β globin chain diseases.

UR - https://www.scopus.com/pages/publications/0023525824

UR - https://www.scopus.com/pages/publications/0023525824#tab=citedBy

U2 - 10.1016/0006-291X(87)90932-6

DO - 10.1016/0006-291X(87)90932-6

M3 - Article

C2 - 3689367

AN - SCOPUS:0023525824

SN - 0006-291X

VL - 148

SP - 694

EP - 700

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Butryic acid analogues augment γ globin gene expression in neonatal erythroid progenitors

Abstract

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