Calcium-sensing mechanism in TRPC5 channels contributing to retardation of neurite outgrowth

Hui Hui, Damian McHugh, Meredith Hannan, Fanning Zeng, Shang Zhong Xu, Saeed ul Hassan Khan, Robert Levenson, David J. Beech, Jamie L. Weiss

Research output: Contribution to journalArticlepeer-review

92 Scopus citations


The calcium- and sodium-permeable transient receptor potential channel TRPC5 has an inhibitory role in neuronal outgrowth but the mechanisms governing its activity are poorly understood. Here we propose a mechanism involving the neuronal calcium sensor-1 (NCS-1) protein. Inhibitory mutants of TRPC5 and NCS-1 enhance neurite outgrowth similarly. Mutant NCS-1 does not inhibit surface-expression of TRPC5 but generally suppresses channel activity, irrespective of whether it is evoked by carbachol, store depletion, lanthanides or elevated intracellular calcium. NCS-1 and TRPC5 are in the same protein complex in rat brain and NCS-1 directly binds to the TRPC5 C-terminus. The data suggest protein-protein interaction between NCS-1 and TRPC5, and involvement of this protein complex in retardation of neurite outgrowth.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalJournal of Physiology
Issue number1
StatePublished - Apr 2006

All Science Journal Classification (ASJC) codes

  • Physiology


Dive into the research topics of 'Calcium-sensing mechanism in TRPC5 channels contributing to retardation of neurite outgrowth'. Together they form a unique fingerprint.

Cite this