Calorie restriction, but not Roux-en-Y gastric bypass surgery, increases [3H] PK11195 binding in a rat model of obesity

John Hamilton, Cynthia Nguyen, Margaret McAvoy, Nicole Roeder, Brittany Richardson, Teresa Quattrin, Andras Hajnal, Panayotis K. Thanos

Research output: Contribution to journalArticlepeer-review


Roux-en-Y gastric bypass surgery (RYGB) remains an effective weight-loss method used to treat obesity. While it is successful in combating obesity, there are many lingering questions related to the changes in the brain following RYGB surgery, one of them being its effects on neuroinflammation. While it is known that chronic high-fat diet (HFD) contributes to obesity and neuroinflammation, it remains to be understood whether bariatric surgery can ameliorate diet-induced inflammatory responses. To examine this, rats were assigned to either a normal diet (ND) or a HFD for 8 weeks. Rats fed a HFD were split into the following groups: sham surgery with ad libitum access to HFD (sham-HF); sham surgery with calorie-restricted HFD (sham-FR); RYGB surgery with ad libitum access to HFD (RYGB). Following sham or RYGB surgeries, rats were maintained on their diets for 9 weeks before being euthanized. [3H] PK11195 autoradiography was then performed on fresh–frozen brain tissue in order to measure activated microglia. Sham-FR rats showed increased [3H] PK11195 binding in the amygdala (63%), perirhinal (60%), and ectorhinal cortex (53%) compared with the ND rats. Obese rats who had the RYGB surgery did not show this increased inflammatory effect. Since the sham-FR and RYGB rats were fed the same amount of HFD, the surgery itself seems responsible for this attenuation in [3H] PK11195 binding. We speculate that calorie restriction following obese conditions may be seen as a stressor and contribute to inflammation in the brain. Further research is needed to verify this mechanism.

Original languageEnglish (US)
Article numbere22258
Issue number2
StatePublished - Mar 2023

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience


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