TY - GEN
T1 - Calorimetric sensing system for real-time urea and creatinine measurements
AU - Lai, Son Vu Hoang
AU - Tadigadapa, Srinivas
PY - 2012
Y1 - 2012
N2 - A high-sensitivity, calorimetric, point-of-care device for automated bedside urine testing based on a micromachined thermal sensor is presented. Immobilized urease and creatinine iminohydrolase enzymes on thin (25 μm) Kapton® films are used to measure the catalytic calorimetric output as a function of concentration. The sensor design locates the active Kapton® film in close proximity to the quartz temperature sensor thereby providing an efficient heat coupling between the two. Measurements of urea concentration at room temperature using the continuous flow set-up and creatinine in open chamber setup at 37 °C are presented. Concentrations of 50 mM for urea and 100 μM for creatinine have been measured. The paper presents the first non-contact, continuous flow thermal sensing measurements. The unique sensor design allows for enzyme immobilized Kapton® film as an easy to swap disposable cartridge and represents a significant advancement for clinical diagnostic application.
AB - A high-sensitivity, calorimetric, point-of-care device for automated bedside urine testing based on a micromachined thermal sensor is presented. Immobilized urease and creatinine iminohydrolase enzymes on thin (25 μm) Kapton® films are used to measure the catalytic calorimetric output as a function of concentration. The sensor design locates the active Kapton® film in close proximity to the quartz temperature sensor thereby providing an efficient heat coupling between the two. Measurements of urea concentration at room temperature using the continuous flow set-up and creatinine in open chamber setup at 37 °C are presented. Concentrations of 50 mM for urea and 100 μM for creatinine have been measured. The paper presents the first non-contact, continuous flow thermal sensing measurements. The unique sensor design allows for enzyme immobilized Kapton® film as an easy to swap disposable cartridge and represents a significant advancement for clinical diagnostic application.
UR - http://www.scopus.com/inward/record.url?scp=84873951218&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873951218&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2012.6411359
DO - 10.1109/ICSENS.2012.6411359
M3 - Conference contribution
AN - SCOPUS:84873951218
SN - 9781457717659
T3 - Proceedings of IEEE Sensors
BT - IEEE SENSORS 2012 - Proceedings
T2 - 11th IEEE SENSORS 2012 Conference
Y2 - 28 October 2012 through 31 October 2012
ER -