TY - JOUR
T1 - Capillary electrophoresis of single cells
T2 - observation of two compartments of neurotransmitter vesicles
AU - Kristensen, Helle K.
AU - Lau, Yau Y.
AU - Ewing, Andrew G.
N1 - Funding Information:
This work was supported, in part, by the National Institutes of Health, the National Science Foundation, and the Office of Naval Research. A.G.E. is a Camille and Henry Dreyfus Teacher-Scholar. Discussions with Prof. Steen Hansen and support to H.K.K. are gratefully acknowledged.
PY - 1994/3
Y1 - 1994/3
N2 - Capillary electrophoresis has been used to directly identify and measure the neurotransmitter, dopamine, in two vesicular compartments in a single nerve cell of Planorbis corneus. Dopamine in the cytoplasm and in easily released transmitter vesicles was separated from dopamine in what are apparently non-functional storage vesicles. In this method, the two peaks in the electropherogram attributed to dopamine were differentiated based on cell lyse time in a non-physiological buffer. The measurements presented here suggest that of the total dopamine present in the cell: 24% is in the cytoplasmic and easily released compartments and 76% is more centrally located, perhaps in a reserve compartment, in the cell. This methodology provides the means to determine molecular species in subcellular compartments and should allow kinetic parameters associated with membrane lysing to be evaluated at single nerve cells.
AB - Capillary electrophoresis has been used to directly identify and measure the neurotransmitter, dopamine, in two vesicular compartments in a single nerve cell of Planorbis corneus. Dopamine in the cytoplasm and in easily released transmitter vesicles was separated from dopamine in what are apparently non-functional storage vesicles. In this method, the two peaks in the electropherogram attributed to dopamine were differentiated based on cell lyse time in a non-physiological buffer. The measurements presented here suggest that of the total dopamine present in the cell: 24% is in the cytoplasmic and easily released compartments and 76% is more centrally located, perhaps in a reserve compartment, in the cell. This methodology provides the means to determine molecular species in subcellular compartments and should allow kinetic parameters associated with membrane lysing to be evaluated at single nerve cells.
UR - http://www.scopus.com/inward/record.url?scp=0028202062&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028202062&partnerID=8YFLogxK
U2 - 10.1016/0165-0270(94)90009-4
DO - 10.1016/0165-0270(94)90009-4
M3 - Article
C2 - 7914252
AN - SCOPUS:0028202062
SN - 0165-0270
VL - 51
SP - 183
EP - 188
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 2
ER -