Capillary Electrophoresis with indirect amperometric detection

Teresa M. Olefirowicz; Andrew G. Ewing

doi:10.1016/S0021-9673(00)97015-8

Capillary Electrophoresis with indirect amperometric detection

Teresa M. Olefirowicz, Andrew G. Ewing

Chemistry

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

The use of indirect amperometric detection with capillary electrophoresis is demonstrated. The system consists of a porous glass coupler which allows amperometric detection at a carbon fiber electrode placed in the end of the capillary. 3,4-Dihydroxybenzylamine is added to the buffer system as a continuously eluting electrophore. Indirect amperometric detection in 9-μm I.D. capillaries provides detection limits as low as 380 attomole for the amino acid arginine. Finally, both direct and indirect amperometric detection can be accomplished simultaneously.

Original language	English (US)
Pages (from-to)	713-719
Number of pages	7
Journal	Journal of Chromatography A
Volume	499
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(00)97015-8
State	Published - Jan 19 1990

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(00)97015-8

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title = "Capillary Electrophoresis with indirect amperometric detection",

abstract = "The use of indirect amperometric detection with capillary electrophoresis is demonstrated. The system consists of a porous glass coupler which allows amperometric detection at a carbon fiber electrode placed in the end of the capillary. 3,4-Dihydroxybenzylamine is added to the buffer system as a continuously eluting electrophore. Indirect amperometric detection in 9-μm I.D. capillaries provides detection limits as low as 380 attomole for the amino acid arginine. Finally, both direct and indirect amperometric detection can be accomplished simultaneously.",

author = "Olefirowicz, {Teresa M.} and Ewing, {Andrew G.}",

note = "Funding Information: This material is based on work supported by the National Institutes of Health under Grant No. GM37621-03 and by Shell Development, Beckman Instruments, Sterling Research Group, Monsanto Company and Lilly Research. Andrew G. Ewing is the recipient of a Presidential Young Investigator Award (CHE-8657193) and is an Alfred P. Sloan Research Fellow.",

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T1 - Capillary Electrophoresis with indirect amperometric detection

AU - Olefirowicz, Teresa M.

AU - Ewing, Andrew G.

N1 - Funding Information: This material is based on work supported by the National Institutes of Health under Grant No. GM37621-03 and by Shell Development, Beckman Instruments, Sterling Research Group, Monsanto Company and Lilly Research. Andrew G. Ewing is the recipient of a Presidential Young Investigator Award (CHE-8657193) and is an Alfred P. Sloan Research Fellow.

PY - 1990/1/19

Y1 - 1990/1/19

N2 - The use of indirect amperometric detection with capillary electrophoresis is demonstrated. The system consists of a porous glass coupler which allows amperometric detection at a carbon fiber electrode placed in the end of the capillary. 3,4-Dihydroxybenzylamine is added to the buffer system as a continuously eluting electrophore. Indirect amperometric detection in 9-μm I.D. capillaries provides detection limits as low as 380 attomole for the amino acid arginine. Finally, both direct and indirect amperometric detection can be accomplished simultaneously.

AB - The use of indirect amperometric detection with capillary electrophoresis is demonstrated. The system consists of a porous glass coupler which allows amperometric detection at a carbon fiber electrode placed in the end of the capillary. 3,4-Dihydroxybenzylamine is added to the buffer system as a continuously eluting electrophore. Indirect amperometric detection in 9-μm I.D. capillaries provides detection limits as low as 380 attomole for the amino acid arginine. Finally, both direct and indirect amperometric detection can be accomplished simultaneously.

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JF - Journal of Chromatography A

IS - C

ER -

Capillary Electrophoresis with indirect amperometric detection

Abstract

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