Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum

Wayne M. Jurick; Ivana Vico; Verneta L. Gaskins; Kari A. Peter; Eunhee Park; Wojciech J. Janisiewicz; William S. Conway

doi:10.1080/03235408.2012.657893

Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum

Wayne M. Jurick, Ivana Vico, Verneta L. Gaskins, Kari A. Peter, Eunhee Park, Wojciech J. Janisiewicz, William S. Conway

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The influence of carbon, nitrogen and pH on polygalacturonase (PG) activity produced by Penicillium expansum were investigated. P. expansum mycelial growth was greatest on lyophilized lyophilised fruit tissue and the highest PG activity occurred in apple pectin medium. Nitrogen source influenced PG activity and was highest with ammonia while the greatest mycelial mass was supported by glutamate or glutamine. PG activity and mycelial mass peaked 5 five days after inoculation as polyuronide content decreased and the pH and ammonium levels increased in apple pectin medium. A single active PG isozyme with an isoelectric point of ~7.6 was produced in apple pectin medium and a partial cDNA clone was obtained that was most homologous to the pggII gene from Penicillium. griseoroseum. The results from this study indicate that P. expansum can modulate the activity of PG in response to nutrient sources and ambient pH through signalling pathways that modulate nutrient acquisition, uptake and metabolism.

Original language	English (US)
Pages (from-to)	1101-1114
Number of pages	14
Journal	Archives of Phytopathology and Plant Protection
Volume	45
Issue number	9
DOIs	https://doi.org/10.1080/03235408.2012.657893
State	Published - May 2012

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science

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10.1080/03235408.2012.657893

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title = "Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum",

abstract = "The influence of carbon, nitrogen and pH on polygalacturonase (PG) activity produced by Penicillium expansum were investigated. P. expansum mycelial growth was greatest on lyophilized lyophilised fruit tissue and the highest PG activity occurred in apple pectin medium. Nitrogen source influenced PG activity and was highest with ammonia while the greatest mycelial mass was supported by glutamate or glutamine. PG activity and mycelial mass peaked 5 five days after inoculation as polyuronide content decreased and the pH and ammonium levels increased in apple pectin medium. A single active PG isozyme with an isoelectric point of ~7.6 was produced in apple pectin medium and a partial cDNA clone was obtained that was most homologous to the pggII gene from Penicillium. griseoroseum. The results from this study indicate that P. expansum can modulate the activity of PG in response to nutrient sources and ambient pH through signalling pathways that modulate nutrient acquisition, uptake and metabolism.",

author = "Jurick, {Wayne M.} and Ivana Vico and Gaskins, {Verneta L.} and Peter, {Kari A.} and Eunhee Park and Janisiewicz, {Wojciech J.} and Conway, {William S.}",

note = "Funding Information: This work was supported by USDA project plan #1275-42430-008-00D. Mention of trade names or commercial products in the publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.",

year = "2012",

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T1 - Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum

AU - Jurick, Wayne M.

AU - Vico, Ivana

AU - Gaskins, Verneta L.

AU - Peter, Kari A.

AU - Park, Eunhee

AU - Janisiewicz, Wojciech J.

AU - Conway, William S.

N1 - Funding Information: This work was supported by USDA project plan #1275-42430-008-00D. Mention of trade names or commercial products in the publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

PY - 2012/5

Y1 - 2012/5

N2 - The influence of carbon, nitrogen and pH on polygalacturonase (PG) activity produced by Penicillium expansum were investigated. P. expansum mycelial growth was greatest on lyophilized lyophilised fruit tissue and the highest PG activity occurred in apple pectin medium. Nitrogen source influenced PG activity and was highest with ammonia while the greatest mycelial mass was supported by glutamate or glutamine. PG activity and mycelial mass peaked 5 five days after inoculation as polyuronide content decreased and the pH and ammonium levels increased in apple pectin medium. A single active PG isozyme with an isoelectric point of ~7.6 was produced in apple pectin medium and a partial cDNA clone was obtained that was most homologous to the pggII gene from Penicillium. griseoroseum. The results from this study indicate that P. expansum can modulate the activity of PG in response to nutrient sources and ambient pH through signalling pathways that modulate nutrient acquisition, uptake and metabolism.

AB - The influence of carbon, nitrogen and pH on polygalacturonase (PG) activity produced by Penicillium expansum were investigated. P. expansum mycelial growth was greatest on lyophilized lyophilised fruit tissue and the highest PG activity occurred in apple pectin medium. Nitrogen source influenced PG activity and was highest with ammonia while the greatest mycelial mass was supported by glutamate or glutamine. PG activity and mycelial mass peaked 5 five days after inoculation as polyuronide content decreased and the pH and ammonium levels increased in apple pectin medium. A single active PG isozyme with an isoelectric point of ~7.6 was produced in apple pectin medium and a partial cDNA clone was obtained that was most homologous to the pggII gene from Penicillium. griseoroseum. The results from this study indicate that P. expansum can modulate the activity of PG in response to nutrient sources and ambient pH through signalling pathways that modulate nutrient acquisition, uptake and metabolism.

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Carbon, nitrogen and pH regulate the production and activity of a polygalacturonase isozyme produced by Penicillium expansum

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