Carboxymethyldextran Lactone: A Preactivated Polymer for Amine Conjugations

Ned D. Heindel; Michael A. Kauffman; Eric K. Akyea; Stephanie A. Engel; Michael F. Frey; C. Jeffrey Lacey; Roger A. Egolf

doi:10.1021/bc00025a013

Carboxymethyldextran Lactone: A Preactivated Polymer for Amine Conjugations

Ned D. Heindel, Michael A. Kauffman, Eric K. Akyea, Stephanie A. Engel, Michael F. Frey, C. Jeffrey Lacey, Roger A. Egolf

Penn State Lehigh Valley

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The linking of amino haptens to carboxymethyldextran (CMD) requires carboxyl activation, for example, via carbodiimides. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be conveniently lactonized by heating in inert solvents, and this carboxymethyldextran lactone (CDL) can be employed directly for amine conjugation.

Original language	English (US)
Pages (from-to)	98-100
Number of pages	3
Journal	Bioconjugate Chemistry
Volume	5
Issue number	1
DOIs	https://doi.org/10.1021/bc00025a013
State	Published - Jan 1 1994

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/bc00025a013

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abstract = "The linking of amino haptens to carboxymethyldextran (CMD) requires carboxyl activation, for example, via carbodiimides. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be conveniently lactonized by heating in inert solvents, and this carboxymethyldextran lactone (CDL) can be employed directly for amine conjugation.",

author = "Heindel, {Ned D.} and Kauffman, {Michael A.} and Akyea, {Eric K.} and Engel, {Stephanie A.} and Frey, {Michael F.} and Lacey, {C. Jeffrey} and Egolf, {Roger A.}",

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T2 - A Preactivated Polymer for Amine Conjugations

AU - Heindel, Ned D.

AU - Kauffman, Michael A.

AU - Akyea, Eric K.

AU - Engel, Stephanie A.

AU - Frey, Michael F.

AU - Lacey, C. Jeffrey

AU - Egolf, Roger A.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The linking of amino haptens to carboxymethyldextran (CMD) requires carboxyl activation, for example, via carbodiimides. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be conveniently lactonized by heating in inert solvents, and this carboxymethyldextran lactone (CDL) can be employed directly for amine conjugation.

AB - The linking of amino haptens to carboxymethyldextran (CMD) requires carboxyl activation, for example, via carbodiimides. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be conveniently lactonized by heating in inert solvents, and this carboxymethyldextran lactone (CDL) can be employed directly for amine conjugation.

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Carboxymethyldextran Lactone: A Preactivated Polymer for Amine Conjugations

Abstract

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