TY - JOUR
T1 - Catalytic antibodies
T2 - Perusing combinatorial libraries
AU - Posner, Bruce
AU - Smiley, Jeffrey
AU - Lee, Irene
AU - Benkovic, Stephen
N1 - Funding Information:
My thanks to the Cancer Kesearch Campaign for financial support, Julia Turner for helpful advice, and Tim Hunt
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994/4
Y1 - 1994/4
N2 - Combinatorial libraries are a promising alternative for isolating catalytic antibodies produced by the immune system in response to the transition-state analog of a given reaction. Large, diverse panels of antibodies with high affinity for the transition-state analog can be isolated using screening or selection approaches. Furthermore, we have estimated that nucleotide sequences that bear close similarity to the sequence for a known catalytic antibody occur in combination at frequencies sufficient for their detection in such libraries.
AB - Combinatorial libraries are a promising alternative for isolating catalytic antibodies produced by the immune system in response to the transition-state analog of a given reaction. Large, diverse panels of antibodies with high affinity for the transition-state analog can be isolated using screening or selection approaches. Furthermore, we have estimated that nucleotide sequences that bear close similarity to the sequence for a known catalytic antibody occur in combination at frequencies sufficient for their detection in such libraries.
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U2 - 10.1016/0968-0004(94)90273-9
DO - 10.1016/0968-0004(94)90273-9
M3 - Article
C2 - 8016861
AN - SCOPUS:0028178511
SN - 0968-0004
VL - 19
SP - 145
EP - 150
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
IS - 4
ER -