CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation

Mark A. Daniels; Lesley Devine; Joseph D. Miller; Janice M. Moser; Aron E. Lukacher; John D. Altman; Paula Kavathas; Kristin A. Hogquist; Stephen C. Jameson

doi:10.1016/S1074-7613(01)00252-7

CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation

Mark A. Daniels, Lesley Devine, Joseph D. Miller, Janice M. Moser, Aron E. Lukacher, John D. Altman, Paula Kavathas, Kristin A. Hogquist, Stephen C. Jameson

Research output: Contribution to journal › Article › peer-review

171 Scopus citations

Abstract

CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8⁺ T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.

Original language	English (US)
Pages (from-to)	1051-1061
Number of pages	11
Journal	Immunity
Volume	15
Issue number	6
DOIs	https://doi.org/10.1016/S1074-7613(01)00252-7
State	Published - 2001

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1074-7613(01)00252-7

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title = "CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation",

abstract = "CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8+ T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.",

author = "Daniels, {Mark A.} and Lesley Devine and Miller, {Joseph D.} and Moser, {Janice M.} and Lukacher, {Aron E.} and Altman, {John D.} and Paula Kavathas and Hogquist, {Kristin A.} and Jameson, {Stephen C.}",

note = "Funding Information: We thank Royce Robinson-Lawler and Bruce Walcheck for help in establishing the adhesion assays, and Matt Mescher and members of the Jameson and Hogquist labs for helpful discussions. We also wish to thank Ellis Reinherz and Jamey Marth for communicating their findings ahead of publication. This work was supported in part by the following grants from the National Institutes of Health: R01 AI38903 (S.C.J.) and R01 CA48115 (P.K.). M.A.D. was supported by an NIH Immunology Training Grant (T32 AI07313).",

year = "2001",

doi = "10.1016/S1074-7613(01)00252-7",

language = "English (US)",

volume = "15",

pages = "1051--1061",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "6",

}

TY - JOUR

T1 - CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation

AU - Daniels, Mark A.

AU - Devine, Lesley

AU - Miller, Joseph D.

AU - Moser, Janice M.

AU - Lukacher, Aron E.

AU - Altman, John D.

AU - Kavathas, Paula

AU - Hogquist, Kristin A.

AU - Jameson, Stephen C.

N1 - Funding Information: We thank Royce Robinson-Lawler and Bruce Walcheck for help in establishing the adhesion assays, and Matt Mescher and members of the Jameson and Hogquist labs for helpful discussions. We also wish to thank Ellis Reinherz and Jamey Marth for communicating their findings ahead of publication. This work was supported in part by the following grants from the National Institutes of Health: R01 AI38903 (S.C.J.) and R01 CA48115 (P.K.). M.A.D. was supported by an NIH Immunology Training Grant (T32 AI07313).

PY - 2001

Y1 - 2001

N2 - CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8+ T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.

AB - CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8+ T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.

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AN - SCOPUS:0035693584

SN - 1074-7613

VL - 15

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EP - 1061

JO - Immunity

JF - Immunity

IS - 6

ER -

CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation

Abstract

All Science Journal Classification (ASJC) codes

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