TY - CHAP
T1 - Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice
AU - Chang, Fang
AU - Zhang, Zaibao
AU - Jin, Yue
AU - Ma, Hong
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.
AB - Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.
UR - http://www.scopus.com/inward/record.url?scp=84898794584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898794584&partnerID=8YFLogxK
U2 - 10.1007/978-1-4614-9408-9-9
DO - 10.1007/978-1-4614-9408-9-9
M3 - Chapter
C2 - 24395258
SN - 9781461494072
T3 - Methods in Molecular Biology
SP - 203
EP - 216
BT - Methods in Molecular Biology
ER -