@inbook{6cc64f811b214574a8b6316ccd7666e2,
title = "Cell biological analyses of anther morphogenesis and pollen viability in arabidopsis and rice",
abstract = "Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.",
author = "Fang Chang and Zaibao Zhang and Yue Jin and Hong Ma",
year = "2014",
doi = "10.1007/978-1-4614-9408-9_9",
language = "English (US)",
isbn = "9781461494072",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "203--216",
booktitle = "Flower Development",
}