Cell-density-dependent regulation of expression and glycosylation of dopachrome tautomerase/tyrosinase related protein-2

Thomas J. Hornyak, Daniel J. Hayes, Edward B. Ziff

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24 Scopus citations


The expression of the dopachrome tautomerase gent (Dct) and its protein product, tyrosinase-related protein-2, was studied in the cultured, phorbol- ester-dependent murine melanocyte cell line melan-a. Increased cell density was found to stimulate Dct expression both in cells stably transfected with a Dct promoter-lacZ construct and endogenously in nontransfected cells. Increased Dct expression under these conditions corresponds to increased tyrosinase-related protein-2 production. Tyrosinase-related protein-2 was found to exist in two distinct glycoforms with different endoglycosidase sensitivities. Density-dependent expression of tyrosinase-related protein-2 was independent of time of cell growth, cell proliferation, and soluble factors, implying that cell-cell contact is the important determinant governing increased Dct expression under these conditions. Tyrp1 gent expression and tyrosinase-related protein-1 production were also induced under similar conditions. The results show that cell-cell contact between melanocytes induces a coordinated response at both transcriptional and nontranscriptional levels that induces production of the tyrosinase-related proteins that have a significant role in melanization.

Original languageEnglish (US)
Article number5600754
Pages (from-to)106-112
Number of pages7
JournalJournal of Investigative Dermatology
Issue number1
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology


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