TY - JOUR
T1 - Cell density influences hormonal responsiveness but not lipoprotein utilization in cultured bovine luteal cells
AU - Pate, J. L.
AU - Nephew, K. P.
AU - Zarle, G. S.
N1 - Funding Information:
V.3.A. Salaries and research support provided by State and Federal Funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University, Journal Article No. 187-86. * Present address: Animal Science Department, 2029 Fyffe Road, The Ohio State University, Cols., OH 43210, U.S.A. * * Present address: B-308, 3500 Sutherland Avenue, Knox-ville, TN 38919, U.S.A.
PY - 1987/10
Y1 - 1987/10
N2 - The objective of the present study was to investigate the effect of cell density on hormonal responsiveness and lipoprotein utilization by cultured bovine luteal cells. Luteal cells obtained from regularly cycling dairy cows were plated at three culture densities: 0.5 × 106, 1 × 106 and 2 × 106 cells/flask in serum-free Ham's F-12 culture medium, and maintained for 9 days. Basal steroidogenesis was unaffected by cell density, while LH responsiveness was greatest in low density cultures. Progesterone produced in response to LH (10 ng/ml) was greater than control levels throughout the culture period in low density cultures. Luteal cells cultured at medium and high densities became responsive to LH only later in the culture period (days 5 and 9, respectively). In contrast, prostaglandin F2α (PGF2α, 100 ng/ml) was more effective in high density cultures, causing a complete inhibition of LH stimulation and returning progesterone levels to basal values. In low density cultures, treatment with PGF2α + LH resulted in progesterone levels that were not significantly different from LH-treated cultures. There was no effect of cell density on utilization of either low density lipoprotein (LDL) or high density lipoprotein (HDL) for steroidogenesis. However, a synergistic effect of LH with either lipoprotein was observed in low and medium density, but not high density cultures. From these results, it is concluded that culture density can influence the responsiveness of bovine luteal cells to either LH or PGF2α, but has no effect on lipoprotein utilization by these cells.
AB - The objective of the present study was to investigate the effect of cell density on hormonal responsiveness and lipoprotein utilization by cultured bovine luteal cells. Luteal cells obtained from regularly cycling dairy cows were plated at three culture densities: 0.5 × 106, 1 × 106 and 2 × 106 cells/flask in serum-free Ham's F-12 culture medium, and maintained for 9 days. Basal steroidogenesis was unaffected by cell density, while LH responsiveness was greatest in low density cultures. Progesterone produced in response to LH (10 ng/ml) was greater than control levels throughout the culture period in low density cultures. Luteal cells cultured at medium and high densities became responsive to LH only later in the culture period (days 5 and 9, respectively). In contrast, prostaglandin F2α (PGF2α, 100 ng/ml) was more effective in high density cultures, causing a complete inhibition of LH stimulation and returning progesterone levels to basal values. In low density cultures, treatment with PGF2α + LH resulted in progesterone levels that were not significantly different from LH-treated cultures. There was no effect of cell density on utilization of either low density lipoprotein (LDL) or high density lipoprotein (HDL) for steroidogenesis. However, a synergistic effect of LH with either lipoprotein was observed in low and medium density, but not high density cultures. From these results, it is concluded that culture density can influence the responsiveness of bovine luteal cells to either LH or PGF2α, but has no effect on lipoprotein utilization by these cells.
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U2 - 10.1016/0303-7207(87)90173-0
DO - 10.1016/0303-7207(87)90173-0
M3 - Article
C2 - 3478277
AN - SCOPUS:0023267315
SN - 0303-7207
VL - 53
SP - 187
EP - 194
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 3
ER -