TY - JOUR
T1 - Cell relaxation after electrodeformation
T2 - Effect of latrunculin A on cytoskeletal actin
AU - Wong, Pak Kin
AU - Tan, Winny
AU - Ho, Chih Ming
N1 - Funding Information:
This work is supported by CMISE through NASA URETI program. The authors would like to thank Ming-Ham Yip for his valuable suggestions in the development of the algorithm for the relaxation spectrum analysis.
PY - 2005/3
Y1 - 2005/3
N2 - Precise measurement of the mechanical properties of a cell provides useful information about its structural organization and physiological state. It is interesting to understand the effect of individual components on the mechanical properties of the entire cell. In this study, we investigate the influence of the cytoskeletal actin on the viscoelastic properties of a cell. Actin-specific agents, including latrunculin A and jasplakinolide, are used to alter the organization of the cytoskeletal actin. Brassica oleracea protoplasts are treated with the drugs and deformed under an external electric potential. The relaxation processes of single protoplasts after electrodeformation are measured. The data are analyzed by a model-independent spectrum recovery algorithm. Two distinct characteristic time constants are obtained from the relaxation spectra. Treatment with latrunculin A increases both of the relaxation time constants. The longest relaxation times for control, latrunculin A treated, and jasplakinolide treated cells are determined to be 0.28, 1.0, and 0.21s, respectively.
AB - Precise measurement of the mechanical properties of a cell provides useful information about its structural organization and physiological state. It is interesting to understand the effect of individual components on the mechanical properties of the entire cell. In this study, we investigate the influence of the cytoskeletal actin on the viscoelastic properties of a cell. Actin-specific agents, including latrunculin A and jasplakinolide, are used to alter the organization of the cytoskeletal actin. Brassica oleracea protoplasts are treated with the drugs and deformed under an external electric potential. The relaxation processes of single protoplasts after electrodeformation are measured. The data are analyzed by a model-independent spectrum recovery algorithm. Two distinct characteristic time constants are obtained from the relaxation spectra. Treatment with latrunculin A increases both of the relaxation time constants. The longest relaxation times for control, latrunculin A treated, and jasplakinolide treated cells are determined to be 0.28, 1.0, and 0.21s, respectively.
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U2 - 10.1016/j.jbiomech.2004.04.008
DO - 10.1016/j.jbiomech.2004.04.008
M3 - Article
C2 - 15652551
AN - SCOPUS:12344279082
SN - 0021-9290
VL - 38
SP - 529
EP - 535
JO - Journal of Biomechanics
JF - Journal of Biomechanics
IS - 3
ER -