TY - JOUR
T1 - Cell type- and tissue context-dependent nuclear distribution of human Ago2
AU - Sharma, Nishi R.
AU - Wang, Xiaohong
AU - Majerciak, Vladimir
AU - Ajiro, Masahiko
AU - Kruhlak, Michael
AU - Meyers, Craig
AU - Zheng, Zhi Ming
N1 - Funding Information:
This study was supported by the NCI Investigator-initiated Intramural Research Project 1ZIASC010357-15, the Center for Cancer Research, NCI, National Institutes of Health (to Z. M. Z.). This work was also supported partially by National Institutes of Health Grant U01 CA179724 (to C. M.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the author and does not necessarily represent the official views of the National Institutes of Health.
PY - 2016/1/29
Y1 - 2016/1/29
N2 - Argonaute-2 protein (Ago2), a major component of RNA-induced silencing complex (RISC), has been viewed as a cytoplasmic protein. In this study, we demonstrated by immunofluorescence confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a minimal level of nuclear distribution in continuous cell lines such as HeLa and HaCaT cells. Oncogenic human papillomavirus type 16 (HPV16) or type 18 (HPV18) infection of the keratinocytes does not affect the nuclear Ago2 distribution. Examination of human tissues reveals that Ago2 exhibits primarily as a nuclear protein in skin, normal cervix, and cervical cancer tissues, but not in larynx. Together, our data provide the first convincing evidence that the subcellular distribution of Ago2 occurs in a cell type- and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression.
AB - Argonaute-2 protein (Ago2), a major component of RNA-induced silencing complex (RISC), has been viewed as a cytoplasmic protein. In this study, we demonstrated by immunofluorescence confocal microscopy that Ago2 is distributed mainly as a nuclear protein in primary human foreskin keratinocytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a minimal level of nuclear distribution in continuous cell lines such as HeLa and HaCaT cells. Oncogenic human papillomavirus type 16 (HPV16) or type 18 (HPV18) infection of the keratinocytes does not affect the nuclear Ago2 distribution. Examination of human tissues reveals that Ago2 exhibits primarily as a nuclear protein in skin, normal cervix, and cervical cancer tissues, but not in larynx. Together, our data provide the first convincing evidence that the subcellular distribution of Ago2 occurs in a cell type- and tissue context-dependent manner and may correlate with its various functions in regulation of gene expression.
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U2 - 10.1074/jbc.C115.695049
DO - 10.1074/jbc.C115.695049
M3 - Article
C2 - 26699195
AN - SCOPUS:84957031487
SN - 0021-9258
VL - 291
SP - 2302
EP - 2309
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 5
ER -