TY - JOUR
T1 - Cellular and proteomic events associated with the localized formation of smut-gall during Zizania latifolia–Ustilago esculenta interaction
AU - Jose, Robinson C.
AU - Bengyella, Louis
AU - Handique, Pratap J.
AU - Talukdar, Narayan C.
N1 - Funding Information:
This work was supported by DST (Department of Science and Technology) INSPIRE (Innovation in science pursuit for inspired research) Govt. of INDIA as fellowship (IF 120609 ). We thank NCBS C-CAMP Bangalore for analyzing 2DE and SDS PAGE separated protein spots and bands in ESI based LC-MS/MS. Our sincere thanks also go to the lab members of CMBL and MRD of IBSD for their support and encouragement.
Funding Information:
This work was supported by DST (Department of Science and Technology) INSPIRE (Innovation in science pursuit for inspired research) Govt. of INDIA as fellowship (IF120609). We thank NCBS C-CAMP Bangalore for analyzing 2DE and SDS PAGE separated protein spots and bands in ESI based LC-MS/MS. Our sincere thanks also go to the lab members of CMBL and MRD of IBSD for their support and encouragement.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/1
Y1 - 2019/1
N2 - The perennial wild rice Zizania latifolia is confined in the swampy habitat and wetland of the Indo-Burma biodiversity hotspot of India and infection by the biotrophic fungus Ustilago esculenta is hallmarked by swellings that develop to form localized smut-gall at the topmost internodal region. The cellular and proteomic events involved in the non-systemic colonization of Z. latifolia by U. esculenta leading to smut-gall formation is poorly understood. Proteins were extracted from the smut-gall region at the topmost internodal region below the apical meristematic tissue from the infected and uninfected parts of Z. latifolia. By combining transmission electron microscopy (TEM) and fluorescent microscopy (FM), we showed that U. esculenta hyphal morphological transitions and movement occurred both intercellularly and intracellularly while sporulation occurred intracellularly in selective cells. Following proteome profiling using two dimensional SDS-PAGE at different phenological phases of smut-gall development and U. esculenta infection, differentially expressed proteins bands and their relative abundance were detected and subjected to liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Importantly, the fungus explores at least 7 metabolic pathways and 5 major biological processes to subdue the host defense and thrive successfully on Z. latifolia. The fungus U. esculenta produces proteases and energy acquisition proteins those enhance it's defensive and survival mode in the host. The identified differentially regulated proteins shed-light into why inflorescence is being replaced by bulbous smut-gall at late stages of the disease, as well as the development of resistance in some Z. latifolia plants against U. esculenta infection.
AB - The perennial wild rice Zizania latifolia is confined in the swampy habitat and wetland of the Indo-Burma biodiversity hotspot of India and infection by the biotrophic fungus Ustilago esculenta is hallmarked by swellings that develop to form localized smut-gall at the topmost internodal region. The cellular and proteomic events involved in the non-systemic colonization of Z. latifolia by U. esculenta leading to smut-gall formation is poorly understood. Proteins were extracted from the smut-gall region at the topmost internodal region below the apical meristematic tissue from the infected and uninfected parts of Z. latifolia. By combining transmission electron microscopy (TEM) and fluorescent microscopy (FM), we showed that U. esculenta hyphal morphological transitions and movement occurred both intercellularly and intracellularly while sporulation occurred intracellularly in selective cells. Following proteome profiling using two dimensional SDS-PAGE at different phenological phases of smut-gall development and U. esculenta infection, differentially expressed proteins bands and their relative abundance were detected and subjected to liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Importantly, the fungus explores at least 7 metabolic pathways and 5 major biological processes to subdue the host defense and thrive successfully on Z. latifolia. The fungus U. esculenta produces proteases and energy acquisition proteins those enhance it's defensive and survival mode in the host. The identified differentially regulated proteins shed-light into why inflorescence is being replaced by bulbous smut-gall at late stages of the disease, as well as the development of resistance in some Z. latifolia plants against U. esculenta infection.
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U2 - 10.1016/j.micpath.2018.10.028
DO - 10.1016/j.micpath.2018.10.028
M3 - Article
C2 - 30367966
AN - SCOPUS:85055694023
SN - 0882-4010
VL - 126
SP - 79
EP - 84
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
ER -