TY - JOUR
T1 - Centriole Age Underlies Asynchronous Primary Cilium Growth in Mammalian Cells
AU - Anderson, Charles T.
AU - Stearns, Tim
N1 - Funding Information:
We thank J. Salisbury, H. Hamada, R. Tsien, K. Lee, R. Rohatgi, and P. Beachy for reagents; L. Schneider for staining advice; and A. Vasey and members of the Stearns laboratory for comments on the manuscript. This work was supported by NIH grant GM52022 (T.S.) and National Science Foundation Graduate Research and Stanford Graduate fellowships (C.T.A.).
PY - 2009/9/15
Y1 - 2009/9/15
N2 - Primary cilia are microtubule-based sensory organelles that play important roles in development and disease [1]. They are required for Sonic hedgehog (Shh) [2-4] and platelet-derived growth factor (PDGF) [5] signaling. Primary cilia grow from the older of the two centrioles of the centrosome, referred to as the mother centriole. In cycling cells, the cilium typically grows in G1 and is lost before mitosis, but the regulation of its growth is poorly understood. Centriole duplication at G1/S results in two centrosomes, one with an older mother centriole and one with a new mother centriole, that are segregated in mitosis. Here we report that primary cilia grow asynchronously in sister cells resulting from a mitotic division and that the sister cell receiving the older mother centriole usually grows a primary cilium first. We also show that the signaling proteins inversin [6] and PDGFRα localize asynchronously to sister cell primary cilia and that sister cells respond asymmetrically to Shh. These results suggest that the segregation of differently aged mother centrioles, an asymmetry inherent to every animal cell division, can influence the ability of sister cells to respond to environmental signals, potentially altering the behavior or fate of one or both sister cells.
AB - Primary cilia are microtubule-based sensory organelles that play important roles in development and disease [1]. They are required for Sonic hedgehog (Shh) [2-4] and platelet-derived growth factor (PDGF) [5] signaling. Primary cilia grow from the older of the two centrioles of the centrosome, referred to as the mother centriole. In cycling cells, the cilium typically grows in G1 and is lost before mitosis, but the regulation of its growth is poorly understood. Centriole duplication at G1/S results in two centrosomes, one with an older mother centriole and one with a new mother centriole, that are segregated in mitosis. Here we report that primary cilia grow asynchronously in sister cells resulting from a mitotic division and that the sister cell receiving the older mother centriole usually grows a primary cilium first. We also show that the signaling proteins inversin [6] and PDGFRα localize asynchronously to sister cell primary cilia and that sister cells respond asymmetrically to Shh. These results suggest that the segregation of differently aged mother centrioles, an asymmetry inherent to every animal cell division, can influence the ability of sister cells to respond to environmental signals, potentially altering the behavior or fate of one or both sister cells.
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U2 - 10.1016/j.cub.2009.07.034
DO - 10.1016/j.cub.2009.07.034
M3 - Article
C2 - 19682908
AN - SCOPUS:69949142444
SN - 0960-9822
VL - 19
SP - 1498
EP - 1502
JO - Current Biology
JF - Current Biology
IS - 17
ER -