Challenges and triumphs in cryo-electron tomography

Ryan K. Hylton, Matthew T. Swulius

Research output: Contribution to journalReview articlepeer-review

33 Scopus citations

Abstract

Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high. Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to go before declaring victory. Despite its potential, cryo-electron tomography possesses many inherent challenges. How do we image through thick cell samples, and possibly even tissue? How do we identify a protein of interest amidst the noisy, crowded environment of the cytoplasm? How do we target specific moments of a dynamic cellular process for tomographic imaging? In this review, we cover the history of cryo-electron tomography and how it came to be, roughly speaking, as well as the many approaches that have been developed to overcome its intrinsic limitations.

Original languageEnglish (US)
Article number102959
JournaliScience
Volume24
Issue number9
DOIs
StatePublished - Sep 24 2021

All Science Journal Classification (ASJC) codes

  • General

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