TY - CHAP
T1 - Challenges in bio-fabrication of organoid cultures
AU - Peng, Weijie
AU - Datta, Pallab
AU - Wu, Yang
AU - Dey, Madhuri
AU - Ayan, Bugra
AU - Dababneh, Amer
AU - Ozbolat, Ibrahim T.
N1 - Publisher Copyright:
© Springer International Publishing AG, part of Springer Nature 2018.
PY - 2018
Y1 - 2018
N2 - Three-dimensional (3D) organoids have shown advantages in cell culture over traditional two-dimensional (2D) culture, and have great potential in various applications of tissue engineering. However, there are limitations in current organoid fabrication technologies, such as uncontrolled size, poor reproductively, and inadequate complexity of organoids. In this chapter, we present the existing techniques and discuss the major challenges for 3D organoid biofabrication. Future perspectives on organoid bioprinting are also discussed, where bioprinting technologies are expected to make a major contribution in organoid fabrication, such as realizing mass production and constructing complex heterotypic tissues, and thus further advance the translational application of organoids in tissue engineering and regenerative medicine as well drug testing and pharmaceutics.
AB - Three-dimensional (3D) organoids have shown advantages in cell culture over traditional two-dimensional (2D) culture, and have great potential in various applications of tissue engineering. However, there are limitations in current organoid fabrication technologies, such as uncontrolled size, poor reproductively, and inadequate complexity of organoids. In this chapter, we present the existing techniques and discuss the major challenges for 3D organoid biofabrication. Future perspectives on organoid bioprinting are also discussed, where bioprinting technologies are expected to make a major contribution in organoid fabrication, such as realizing mass production and constructing complex heterotypic tissues, and thus further advance the translational application of organoids in tissue engineering and regenerative medicine as well drug testing and pharmaceutics.
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U2 - 10.1007/5584_2018_216
DO - 10.1007/5584_2018_216
M3 - Chapter
C2 - 29855825
AN - SCOPUS:85057549624
T3 - Advances in Experimental Medicine and Biology
SP - 53
EP - 71
BT - Advances in Experimental Medicine and Biology
PB - Springer New York LLC
ER -