TY - JOUR
T1 - Changes in the relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels
AU - Cyr, Richard J.
AU - Sotak, Michael
AU - Bustos, Mauricio M.
AU - Guiltinan, Mark J.
AU - Fosket, Donald E.
N1 - Funding Information:
CA for his generosity in making a protein electrical elution device which was used in the course of this work. This research was supported by grants PCM82-16035 and DCB86-02712 from the National Science Foundation to D.E.F.
PY - 1987/7/24
Y1 - 1987/7/24
N2 - The relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels varies depending upon the electrophoretic methods used to separate them. When reduced and alkylated rat and carrot tubulin heterodimers were separated by one method, the α-tubulin subunits, identified by means of a highly specific antibody, migrated more slowly than the β-tubulin subunits. However, when separated by another method, the carrot α-subunit migrated more rapidly than its β-subunit, while the relative mobility of rat brain tubulin subunits was unchanged. The two gel systems differ principally in the pH of the separating gel during electrophoresis, suggesting that pH markedly influences the interaction of SDS with the plant, but not the vertebrate α-tubulin.
AB - The relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels varies depending upon the electrophoretic methods used to separate them. When reduced and alkylated rat and carrot tubulin heterodimers were separated by one method, the α-tubulin subunits, identified by means of a highly specific antibody, migrated more slowly than the β-tubulin subunits. However, when separated by another method, the carrot α-subunit migrated more rapidly than its β-subunit, while the relative mobility of rat brain tubulin subunits was unchanged. The two gel systems differ principally in the pH of the separating gel during electrophoresis, suggesting that pH markedly influences the interaction of SDS with the plant, but not the vertebrate α-tubulin.
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U2 - 10.1016/0167-4838(87)90157-9
DO - 10.1016/0167-4838(87)90157-9
M3 - Article
AN - SCOPUS:45949119534
SN - 0167-4838
VL - 914
SP - 28
EP - 34
JO - Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
JF - Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
IS - 1
ER -