Changes in tRNA methylase activity of rat kidney following administration of the carcinogen, dimethylnitrosamine

B. W. Stewart; A. E. Pegg

doi:10.1016/0005-2787(72)90457-1

Changes in tRNA methylase activity of rat kidney following administration of the carcinogen, dimethylnitrosamine

B. W. Stewart, A. E. Pegg

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Abstract

1. 1. Administration of dimethylnitrosamine (40 mg/kg body wt) to rats being fed a diet deficient in protein led to an increase in the tRNA methylase activity of the kidney. Maximum activity occurred 2 days after the dimethylnitrosamine was given and a second peak of activity occurred about 4 days later after which the activity declined to normal values. 2. 2. Kidney tumours are produced in a high yield as a result of this treatment and the tumours which appeared some months later also had a higher tRNA methylase activity than control normal kidney. The enhanced tRNA methylase activity appeared to be a general response of all the enzymes detectable by assay with the tRNAs from yeast and Escherichia coli used as substrates. 3. 3. These results are discussed in terms of the changes in nucleic acid synthesis produced in the kidney by dimethylnitrosamine and studies of the histological development of kidney tumours.

Original language	English (US)
Pages (from-to)	416-424
Number of pages	9
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	281
Issue number	3
DOIs	https://doi.org/10.1016/0005-2787(72)90457-1
State	Published - Oct 27 1972

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General Medicine

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title = "Changes in tRNA methylase activity of rat kidney following administration of the carcinogen, dimethylnitrosamine",

abstract = "1. 1. Administration of dimethylnitrosamine (40 mg/kg body wt) to rats being fed a diet deficient in protein led to an increase in the tRNA methylase activity of the kidney. Maximum activity occurred 2 days after the dimethylnitrosamine was given and a second peak of activity occurred about 4 days later after which the activity declined to normal values. 2. 2. Kidney tumours are produced in a high yield as a result of this treatment and the tumours which appeared some months later also had a higher tRNA methylase activity than control normal kidney. The enhanced tRNA methylase activity appeared to be a general response of all the enzymes detectable by assay with the tRNAs from yeast and Escherichia coli used as substrates. 3. 3. These results are discussed in terms of the changes in nucleic acid synthesis produced in the kidney by dimethylnitrosamine and studies of the histological development of kidney tumours.",

author = "Stewart, {B. W.} and Pegg, {A. E.}",

note = "Funding Information: This research was generously supported by the Medical Research Council (Grant No. 967/252B) and by the Michael Sobell Fellowship of the Cancer Research Campaign.",

year = "1972",

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doi = "10.1016/0005-2787(72)90457-1",

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T1 - Changes in tRNA methylase activity of rat kidney following administration of the carcinogen, dimethylnitrosamine

AU - Stewart, B. W.

AU - Pegg, A. E.

N1 - Funding Information: This research was generously supported by the Medical Research Council (Grant No. 967/252B) and by the Michael Sobell Fellowship of the Cancer Research Campaign.

PY - 1972/10/27

Y1 - 1972/10/27

N2 - 1. 1. Administration of dimethylnitrosamine (40 mg/kg body wt) to rats being fed a diet deficient in protein led to an increase in the tRNA methylase activity of the kidney. Maximum activity occurred 2 days after the dimethylnitrosamine was given and a second peak of activity occurred about 4 days later after which the activity declined to normal values. 2. 2. Kidney tumours are produced in a high yield as a result of this treatment and the tumours which appeared some months later also had a higher tRNA methylase activity than control normal kidney. The enhanced tRNA methylase activity appeared to be a general response of all the enzymes detectable by assay with the tRNAs from yeast and Escherichia coli used as substrates. 3. 3. These results are discussed in terms of the changes in nucleic acid synthesis produced in the kidney by dimethylnitrosamine and studies of the histological development of kidney tumours.

AB - 1. 1. Administration of dimethylnitrosamine (40 mg/kg body wt) to rats being fed a diet deficient in protein led to an increase in the tRNA methylase activity of the kidney. Maximum activity occurred 2 days after the dimethylnitrosamine was given and a second peak of activity occurred about 4 days later after which the activity declined to normal values. 2. 2. Kidney tumours are produced in a high yield as a result of this treatment and the tumours which appeared some months later also had a higher tRNA methylase activity than control normal kidney. The enhanced tRNA methylase activity appeared to be a general response of all the enzymes detectable by assay with the tRNAs from yeast and Escherichia coli used as substrates. 3. 3. These results are discussed in terms of the changes in nucleic acid synthesis produced in the kidney by dimethylnitrosamine and studies of the histological development of kidney tumours.

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Changes in tRNA methylase activity of rat kidney following administration of the carcinogen, dimethylnitrosamine

Abstract

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