Abstract
1. A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. 2. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an M(r) of 100000 was observed. 3. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. 4. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.
Original language | English (US) |
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Pages (from-to) | 167-172 |
Number of pages | 6 |
Journal | Biochemical Journal |
Volume | 233 |
Issue number | 1 |
DOIs | |
State | Published - 1986 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Cell Biology