Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase

G. C. Ness, C. E. Sample, M. Smith, L. C. Pendleton, D. C. Eichler

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


1. A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. 2. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an M(r) of 100000 was observed. 3. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. 4. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalBiochemical Journal
Issue number1
StatePublished - 1986

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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